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Western Blot (WB) (Western Blot analysis of extracts from Hela cells untreated or treated with EGF using MyoD (Phospho-Ser200) antibody)

Rabbit MyoD Polyclonal Antibody | anti-MyoD antibody

Rabbit polyclonal MyoD antibody

Gene Names
MYOD1; PUM; MYF3; MYOD; bHLHc1
Applications
Western Blot
Purity
Purified by immunoaffinity chromatography.
Synonyms
MyoD; Polyclonal Antibody; Rabbit polyclonal MyoD antibody; MyoD antibody; anti-MyoD antibody
Ordering
For Research Use Only!
Host
Rabbit
Clonality
Polyclonal
Purity/Purification
Purified by immunoaffinity chromatography.
Form/Format
Supplied in liquid form in PBS buffer (no MG2+ or Ca2+), pH 7.4, 150 mm NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
319
Applicable Applications for anti-MyoD antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
MyoD antibody was raised in Rabbit using the peptide sequence around phosphorylation site of serine 200 (A-S-S(p)-P-R) derived from Human MyoD as the immunogen.
Cross-Reactivity
Human, Mouse, Rat
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts from Hela cells untreated or treated with EGF using MyoD (Phospho-Ser200) antibody)

Western Blot (WB) (Western Blot analysis of extracts from Hela cells untreated or treated with EGF using MyoD (Phospho-Ser200) antibody)
Product Categories/Family for anti-MyoD antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
34,501 Da
NCBI Official Full Name
MyoD
NCBI Official Synonym Full Names
myogenic differentiation 1
NCBI Official Symbol
MYOD1
NCBI Official Synonym Symbols
PUM; MYF3; MYOD; bHLHc1
NCBI Protein Information
myoblast determination protein 1
UniProt Protein Name
Myoblast determination protein 1
Protein Family
UniProt Gene Name
MYOD1
UniProt Synonym Gene Names
BHLHC1; MYF3; MYOD; bHLHc1; Myf-3

NCBI Description

This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

MyoD: a transcription factor of the basic helix-loop-helix family and the myogenic factors subfamily. Involved in muscle differentiation. Induces fibroblasts to differentiate into myoblasts. It is involved in muscle cell differentiation, and is essential for repair of damaged tissue. It activates its own transcription which may stabilize commitment to myogenesis.

Protein type: DNA-binding; RNA splicing; Transcription factor

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: cytoplasm; nuclear chromatin; nucleoplasm; nucleus; transcription factor complex

Molecular Function: chromatin binding; chromatin DNA binding; nuclear hormone receptor binding; protein binding; protein heterodimerization activity; RNA polymerase II transcription factor activity, enhancer binding

Biological Process: muscle cell fate commitment; muscle development; myotube cell development; positive regulation of muscle cell differentiation; positive regulation of myoblast differentiation; positive regulation of skeletal muscle fiber development; positive regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; regulation of RNA splicing; regulation of transcription from RNA polymerase II promoter; skeletal muscle development

Research Articles on MyoD

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Product Notes

The MyoD myod1 (Catalog #AAA5304941) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MyoD can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the MyoD myod1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MyoD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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