Rabbit MYOC Polyclonal Antibody | anti-MYOC antibody

MYOC Polyclonal Antibody

Cat. Num. AAA3008123

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: MYOC; Polyclonal Antibody; MYOC Polyclonal Antibody; Myocilin; Myocilin 55 kDa subunit; Trabecular meshwork-induced glucocorticoid response protein; GLC1A; TIGR; anti-MYOC antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MYOC polyclonal antibody detects endogenous levels of MYOC protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 504

• Applicable Applications for anti-MYOC antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Recombinant full length Human MYOC.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of MYOC antibody.)

Related Product Information for anti-MYOC antibody

-Myocilin is an extracellular protein expressed in the eye, including the retina, trabecular meshwork and ciliary body. Myocilin can form homomultimers in vivo and can also associate with components of the ECM via interactions with the Hep II domain of FibroPVRL1. In addition, myocilin interacts with myosin regulatory light chain, a component of the myosin motor protein complex. This interaction implies a role for myocilin in the actomyosin system, linking myocilin to the functional status of the trabecular meshwork (TM), which is responsible for controlling the intraocular pressure (IOP). Alterations in functions of the TM may lead to IOP elevation and development of glaucoma, a major cause of blindness. Myocilin is encoded by MYOC (also designated TIGR), a gene that maps to the GLC1A locus on chromosome 1q24.3 and is susceptible to mutations. Mutations in the MYOC gene are specifically linked with primary open angle glaucoma (POAG), a blinding disease characterized by progressive loss of retinal ganglion cells.

NCBI and Uniprot Product Information

• NCBI GI #: 3024209
• UniProt Accession #: Q99972
• Molecular Weight: ~ 65kDa
• NCBI Official Full Name: Myocilin
• UniProt Protein Name: Myocilin
• Protein Family: Myocilin
• UniProt Gene Name: MYOC
• UniProt Synonym Gene Names: GLC1A; TIGR
• UniProt Entry Name: MYOC_HUMAN

Uniprot Description

MYOC: May participate in the obstruction of fluid outflow in the trabecular meshwork. Defects in MYOC are the cause of primary open angle glaucoma type 1A (GLC1A). Primary open angle glaucoma (POAG) is characterized by a specific pattern of optic nerve and visual field defects. The angle of the anterior chamber of the eye is open, and usually the intraocular pressure is increased. The disease is asymptomatic until the late stages, by which time significant and irreversible optic nerve damage has already taken place. Defects in MYOC are a cause of primary congenital glaucoma type 3A (GLC3A). An autosomal recessive form of primary congenital glaucoma (PCG). PCG is characterized by marked increase of intraocular pressure at birth or early choldhood, large ocular globes (buphthalmos) and corneal edema. It results from developmental defects of the trabecular meshwork and anterior chamber angle of the eye that prevent adequate drainage of aqueous humor. MYOC variations may contribute to GLC3A via digenic inheritance with CYP1B1 and/or another locus associated with the disease.

Protein type: Secreted; Secreted, signal peptide; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 1q23-q24

Cellular Component: extracellular matrix; Golgi apparatus; extracellular space; proteinaceous extracellular matrix; mitochondrial outer membrane; rough endoplasmic reticulum; endoplasmic reticulum; cytoplasmic membrane-bound vesicle; mitochondrial inner membrane; mitochondrial intermembrane space; cytoplasmic vesicle; cilium

Molecular Function: protein binding; frizzled binding; fibronectin binding; myosin light chain binding; receptor tyrosine kinase binding

Biological Process: clustering of voltage-gated sodium channels; negative regulation of stress fiber formation; negative regulation of cell-matrix adhesion; skeletal muscle hypertrophy; myelination in the peripheral nervous system; osteoblast differentiation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; negative regulation of Rho protein signal transduction; positive regulation of focal adhesion formation; positive regulation of mitochondrial depolarization; regulation of MAPKKK cascade; positive regulation of stress fiber formation; neurite development; positive regulation of cell migration

Disease: Glaucoma 1, Open Angle, A