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Immunohistochemistry (IHC) (Immunohistochemistry (IHC) analysis of MYL9/MYL12A (P13) pAb in paraffin-embedded human lung carcinoma tissue.)

Rabbit MYL9/MYL12A Polyclonal Antibody | anti-MYL9 antibody

MYL9/MYL12A (P13) Polyclonal Antibody

Gene Names
MYL9; LC20; MLC2; MRLC1; MYRL2; MLC-2C
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
MYL9/MYL12A; Polyclonal Antibody; MYL9/MYL12A (P13) Polyclonal Antibody; Myosin regulatory light polypeptide 9; 20 kDa myosin light chain; Short name=LC20; MLC-2C; Myosin RLC; Myosin regulatory light chain 2; smooth muscle isoform; Myosin regulatory light chain 9; Myosin regulatory light chain MRLC1; MLC2; MRLC1; MYRL2; Myosin regulatory light chain 12A; MLC-2B; nonsarcomeric; Myosin regulatory light chain MRLC3; MYL12A; MLCB; MRLC3; RLC; anti-MYL9 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
MYL9/MYL12A (P13) polyclonal antibody detects endogenous levels of MYL9 and MYL12A protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
118
Applicable Applications for anti-MYL9 antibody
Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
IHC: 1:50-1:200
IF: 1:50-1:200
Immunogen
Synthetic peptide, corresponding to the 10-40 of Human MYL9.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analysis of MYL9/MYL12A (P13) pAb in paraffin-embedded human lung carcinoma tissue.)

Immunohistochemistry (IHC) (Immunohistochemistry (IHC) analysis of MYL9/MYL12A (P13) pAb in paraffin-embedded human lung carcinoma tissue.)
Related Product Information for anti-MYL9 antibody
Myosin regulatory light chains, including MRCL3 (also known as MRLC3 or MLCB), MRLC2 (also known as MLC-B) and MYL9 (also known as LC20, MLC2, MRLC1 or MYRL2), regulate contraction in smooth muscle and non-muscle cells via phosphorylation by myosin light chain kinase (MLCK). Phosphorylation of myosin regulatory light chains, catalyzed by MLCK in the presence of calcium and calmodulin, increases the Actin-activated myosin ATPase activity, thereby regulating the contractile activity. Myosin light chain is also located in striated skeletal muscle, where its function remains undefined.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 20kDa
NCBI Official Full Name
myosin regulatory light polypeptide 9 isoform b
NCBI Official Synonym Full Names
myosin light chain 9
NCBI Official Symbol
MYL9
NCBI Official Synonym Symbols
LC20; MLC2; MRLC1; MYRL2; MLC-2C
NCBI Protein Information
myosin regulatory light polypeptide 9
UniProt Protein Name
Myosin regulatory light polypeptide 9
UniProt Gene Name
MYL9
UniProt Synonym Gene Names
MLC2; MRLC1; MYRL2; LC20
UniProt Entry Name
MYL9_HUMAN

NCBI Description

Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

MRLC1: Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion. Myosin is an hexamer of 2 heavy chains and 4 light chains. Smooth muscle tissues and in some, but not all, nonmuscle cells. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Contractile

Chromosomal Location of Human Ortholog: 20q11.23

Cellular Component: stress fiber; muscle myosin complex; cytosol; Z disc

Molecular Function: structural constituent of muscle; calcium ion binding

Biological Process: axon guidance; muscle contraction; regulation of muscle contraction; ephrin receptor signaling pathway

Research Articles on MYL9

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Product Notes

The MYL9 myl9 (Catalog #AAA3001284) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MYL9/MYL12A (P13) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MYL9/MYL12A can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Immunofluorescence (IF). IHC: 1:50-1:200 IF: 1:50-1:200. Researchers should empirically determine the suitability of the MYL9 myl9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MYL9/MYL12A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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