OPA1 Polyclonal Antibody | anti-OPA1 antibody

Anti-OPA1 Antibody

Cat. Num. AAA178050

• Gene Names: OPA1; NPG; NTG; MGM1; BERHS; largeG; MTDPS14
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: OPA1; Polyclonal Antibody; Anti-OPA1 Antibody; Dynamin-like 120 kDa protein; Dynamin like 120 kDa protein; mitochondrial; form S1; FLJ12460; Juvenile kjer type optic atrophy; KIAA0567; KJER type; Large GTP binding protein; largeG; MGM1; Mitochondrial dynamin like 120 kDa protein; Mitochondrial dynamin like GTPase; NPG; NTG; OAK; OPA 1; opa1; OPA1 gene; OPA1_HUMAN; Optic atrophy 1 (autosomal dominant); OPTIC ATROPHY 1; Optic atrophy 1 gene protein; Optic atrophy 1 homolog (human); Optic atrophy protein 1; Optic atrophy protein 1 homolog; ; optic atrophy 1 (autosomal dominant); anti-OPA1 antibody

• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 960

• Applicable Applications for anti-OPA1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml; Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human OPA1 (919-955aa EDGEKKIKLLTGKRVQLAEDLKKVREIQEKLDAFIEA), different from the related mouse and rat sequences by one amino acid.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- OPA1 Picoband antibody, MBS178050, Western blottingAll lanes: Anti OPA1 (MBS178050) at 0.5ug/mlLane 1: A549 Whole Cell Lysate at 40ugLane 2: SKOV Whole Cell Lysate at 40ugLane 3: SW620 Whole Cell Lysate at 40ugPredicted bind size: 110KDObserved bind size: 95KD )

Immunohistochemistry (IHC)

(Anti- OPA1 Picoband antibody, MBS178050, IHC(P)IHC(P): Mouse Liver Tissue )

Immunohistochemistry (IHC)

(Anti- OPA1 Picoband antibody, MBS178050, IHC(P)IHC(P): Rat Kidney Tissue )

Immunohistochemistry (IHC)

(Anti- OPA1 Picoband antibody, MBS178050, IHC(P)IHC(P): Human Lung Cancer Tissue )

Related Product Information for anti-OPA1 antibody

Description: Rabbit IgG polyclonal antibody for Dynamin-like 120 kDa protein, mitochondrial(OPA1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Background: Dynamin-like 120 kDa protein, mitochondrial is a protein that in humans is encoded by the OPA1 gene. It is mapped to 3q29. This protein regulates mitochondrial fusion and cristae structure in the inner mitochondrial membrane (IMM) and contributes to ATP synthesis and apoptosis. This gene product is a nuclear-encoded mitochondrial protein with similarity to dynamin-related GTPases. It is a component of the mitochondrial network. Mutations in this gene have been associated with optic atrophy type 1, which is a dominantly inherited optic neuropathy resulting in progressive loss of visual acuity, leading in many cases to legal blindness. Multiple transcript variants encoding different isoforms have been found for this gene.

References

1. Patten DA, Wong J, Khacho M, Soubannier V, Mailloux RJ, Pilon-Larose K, MacLaurin JG, Park DS, McBride HM, Trinkle-Mulcahy L, Harper ME, Germain M, Slack RS (Nov 2014). "OPA1-dependent cristae modulation is essential for cellular adaptation to metabolic demand". The EMBO Journal 33 (22): 2676-91. 2. Santarelli R, Rossi R, Scimemi P, Cama E, Valentino ML, La Morgia C, Caporali L, Liguori R, Magnavita V, Monteleone A, Biscaro A, Arslan E, Carelli V (Mar 2015). "OPA1-related auditory neuropathy: site of lesion and outcome of cochlear implantation". Brain 138 (Pt 3): 563-76.

NCBI and Uniprot Product Information

• NCBI GI #: 224831243
• NCBI GeneID: 4976
• NCBI Accession #: NP_056375.2
• NCBI GenBank Nucleotide #: NM_015560.2
• UniProt Accession #: O60313; D3DNW4
• Molecular Weight: 115,884 Da
• NCBI Official Full Name: dynamin-like 120 kDa protein, mitochondrial isoform 1
• NCBI Official Synonym Full Names: OPA1, mitochondrial dynamin like GTPase
• NCBI Official Symbol: OPA1
• NCBI Official Synonym Symbols: NPG; NTG; MGM1; BERHS; largeG; MTDPS14
• NCBI Protein Information: dynamin-like 120 kDa protein, mitochondrial
• UniProt Protein Name: Dynamin-like 120 kDa protein, mitochondrial
• Protein Family: Dynamin-like 120 kDa protein
• UniProt Gene Name: OPA1
• UniProt Synonym Gene Names: KIAA0567
• UniProt Entry Name: OPA1_HUMAN

NCBI Description

This gene product is a nuclear-encoded mitochondrial protein with similarity to dynamin-related GTPases. It is a component of the mitochondrial network. Mutations in this gene have been associated with optic atrophy type 1, which is a dominantly inherited optic neuropathy resulting in progressive loss of visual acuity, leading in many cases to legal blindness. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Uniprot Description

OPA1: a dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space. Defects in OPA1 are the cause of optic atrophy 1 and deafness. Eight splice-variant isoforms have been described.

Protein type: Membrane protein, integral; Hydrolase; Mitochondrial; Apoptosis; EC 3.6.5.5

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: cytoplasm; dendrite; integral to membrane; membrane; mitochondrial crista; mitochondrial inner membrane; mitochondrial intermembrane space; mitochondrial outer membrane; mitochondrion; nucleoplasm

Molecular Function: GTP binding; GTPase activity; magnesium ion binding; protein binding

Biological Process: apoptosis; axon transport of mitochondrion; inner mitochondrial membrane organization and biogenesis; metabolic process; mitochondrial fission; mitochondrial fusion; mitochondrial genome maintenance; mitochondrion organization and biogenesis; positive regulation of neuron maturation; visual perception

Disease: Glaucoma, Normal Tension, Susceptibility To; Optic Atrophy 1; Optic Atrophy With Or Without Deafness, Ophthalmoplegia, Myopathy, Ataxia, And Neuropathy