Rabbit anti-Human MYL3 Polyclonal Antibody | anti-MYL3 antibody

MYL3, NT (MYL3, Myosin light chain 3, Cardiac myosin light chain 1, Myosin light chain 1, slow-twitch muscle B/ventricular isoform, Ventricular/slow twitch myosin alkali light chain) (FITC)

Cat. Num. AAA6323322

• Gene Names: MYL3; CMH8; VLC1; MLC1V; MLC1SB
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MYL3; Polyclonal Antibody; NT (MYL3; Myosin light chain 3; Cardiac myosin light chain 1; Myosin light chain 1; slow-twitch muscle B/ventricular isoform; Ventricular/slow twitch myosin alkali light chain) (FITC); Ventricular/slow twitch myosin alkali light chain; anti-MYL3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-MYL3 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: MYL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human MYL3.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MYL3 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the MYL3 antibody detected the MYL3 protein (arrow).)

Immunohistochemistry (IHC)

(MYL3 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MYL3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-MYL3 antibody

MYL3 encodes myosin light chain 3, an alkali light chain also referred to in the literature as both the ventricular isoform and the slow skeletal muscle isoform. Mutations in MYL3 have been identified as a cause of mid-left ventricular chamber type hypertrophic cardiomyopathy.

Product Categories/Family for anti-MYL3 antibody

Antibodies; Cardiac Markers

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Girolami, F., et al. J. Am. Coll. Cardiol. 55(14):1444-1453(2010). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). McLeod, C.J., et al. Am. Heart J. 158(5):799-805(2009). Kaski, J.P., et al. Circ Cardiovasc Genet 2(5):436-441(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4557777
• NCBI GeneID: 4634
• NCBI Accession #: NP_000249.1
• NCBI GenBank Nucleotide #: NM_000258.2
• UniProt Accession #: P08590; Q9NRS8; B2R534
• Molecular Weight: 21,932 Da
• NCBI Official Full Name: myosin light chain 3
• NCBI Official Synonym Full Names: myosin, light chain 3, alkali; ventricular, skeletal, slow
• NCBI Official Symbol: MYL3
• NCBI Official Synonym Symbols: CMH8; VLC1; MLC1V; MLC1SB
• NCBI Protein Information: myosin light chain 3; CMLC1; cardiac myosin light chain 1; ventricular/slow twitch myosin alkali light chain; myosin light chain 1, slow-twitch muscle B/ventricular isoform; myosin, light polypeptide 3, alkali; ventricular, skeletal, slow
• UniProt Protein Name: Myosin light chain 3
• Protein Family: Myosin
• UniProt Gene Name: MYL3
• UniProt Synonym Gene Names: CMLC1; MLC1SB
• UniProt Entry Name: MYL3_HUMAN

NCBI Description

MYL3 encodes myosin light chain 3, an alkali light chain also referred to in the literature as both the ventricular isoform and the slow skeletal muscle isoform. Mutations in MYL3 have been identified as a cause of mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]

Uniprot Description

MYL3: Regulatory light chain of myosin. Does not bind calcium. Defects in MYL3 are the cause of familial hypertrophic cardiomyopathy type 8 (CMH8). Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. CMH8 inheritance can be autosomal dominant or recessive.

Protein type: Motility/polarity/chemotaxis; Motor

Chromosomal Location of Human Ortholog: 3p21.3-p21.2

Cellular Component: I band; sarcomere; muscle myosin complex; cytosol; A band

Molecular Function: actin monomer binding; structural constituent of muscle; motor activity; myosin II heavy chain binding; calcium ion binding

Biological Process: skeletal muscle development; positive regulation of ATPase activity; metabolic process; regulation of striated muscle contraction; ventricular cardiac muscle morphogenesis; regulation of the force of heart contraction; cardiac muscle contraction; muscle filament sliding

Disease: Cardiomyopathy, Familial Hypertrophic, 8