Rabbit anti-Human MYL2 Polyclonal Antibody | anti-MYL2 antibody

Anti-MYL2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8110023

• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: MYL2; Polyclonal Antibody; Anti-MYL2 Antibody; Rabbit Polyclonal; MYL2 Antibody; Rabbit PAb; Antigen Affinity Purified; anti-MYL2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human MYL2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-MYL2 antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:50-1:200
• Immunogen: E Coli-derived Human MYL2 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human MYL2 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of MYL2 in human skeletal muscle with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of MYL2 in cynomolgus heart with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-MYL2 antibody

Myosin regulatory light chain-2 (MYL2), also called MLC-2, is an ~19kDa sarcomeric protein that belongs to the EF-hand calcium binding protein superfamily and exists as three major isoforms encoded by three distinct genes in mammalian striated muscle. Each of the three different MLC-2 genes (MLC-2f; fast twitch skeletal isoform, MLC-2v; cardiac ventricular and slow twitch skeletal isoform, MLC-2a; cardiac atrial isoform) has a distinct developmental expression pattern in mammals. Genetic loss-of-function studies in mice demonstrated an essential role for cardiac isoforms of MLC-2, MLC-2v and MLC-2a, in cardiac contractile function during early embryogenesis. MYL2 encodes a myosin regulatory light chain (MLC-2V). The myosin regulatory light chains bind, together with the essential light chains, to the flexible neck region of the myosin heavy chain in the hexameric myosin complex and have a structural and regulatory role in muscle contraction. The MYL2 mutations are associated with severe cardiac hypertrophy and sudden cardiac death (SCD).

References

Weterman MA, et al. (2013) Recessive myl2 mutations cause infantile type i muscle fibre disease and cardiomyopathy. Brain 136 (Pt 1): 282-293.Sheikh F, et al. (2015) Functions of myosin light chain-2 (myl2) in cardiac muscle and disease. Gene 569 (1): 14-20.Li Y, et al. (2011) Slow cardiac myosin regulatory light chain 2 (myl2) was down-expressed in chronic heart failure patients. Clin Cardiol 34 (1): 30-34.