Rabbit anti-Human MYH8 Polyclonal Antibody | anti-MYH8 antibody

MYH8 Polyclonal Antibody

Cat. Num. AAA3007317

• Gene Names: MYH8; DA7; MyHC-pn; gtMHC-F; MyHC-peri
• Reactivity: Human
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: MYH8; Polyclonal Antibody; MYH8 Polyclonal Antibody; Myosin-8; Myosin heavy chain 8; Myosin heavy chain; skeletal muscle; perinatal; MyHC-perinatal; anti-MYH8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MYH8 polyclonal antibody detects endogenous levels of MYH8 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1937

• Applicable Applications for anti-MYH8 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human MYH8.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of MYH8 polyclonal antibody at 1:500 dilution Line1:HEPG2 whole cell lysate)

Related Product Information for anti-MYH8 antibody

Actin is a highly conserved protein that is expressed in all eukaryotic cells. Actin filaments can form both stable and labile structures, and are crucial components of microvilli and the contractile apparatus of muscle cells. Myosin is a hexamer of two heavy chains (MHC) and four light chains (MLC) that interact with Actin to generate the force for diverse cellular movements, including cytokinesis, phagocytosis and muscle contraction. MYH3 (Myosin heavy chain 3), also known as muscle embryonic Myosin heavy chain or SMHCE, is a 1, 940 amino acid that localizes to the thick filaments of myofibrils. While highly expressed in fetal skeletal muscle, MYH3 is barely detectable in adult skeletal muscle. Defects in the gene encoding MYH3, which maps to human chromosome 17p13.1, are the cause of distal arthrogryposis type 2B (DA2B).

NCBI and Uniprot Product Information

• NCBI GI #: 153945790
• NCBI GeneID: 4626
• NCBI Accession #: NP_002463.2
• NCBI GenBank Nucleotide #: NP_002463.2
• UniProt Accession #: P13535
• Molecular Weight: ~ 223kDa
• NCBI Official Full Name: myosin-8
• NCBI Official Synonym Full Names: myosin heavy chain 8
• NCBI Official Symbol: MYH8
• NCBI Official Synonym Symbols: DA7; MyHC-pn; gtMHC-F; MyHC-peri
• NCBI Protein Information: myosin-8
• UniProt Protein Name: Myosin-8
• Protein Family: Myosin
• UniProt Gene Name: MYH8
• UniProt Synonym Gene Names: MyHC-perinatal
• UniProt Entry Name: MYH8_HUMAN

NCBI Description

Myosins are actin-based motor proteins that function in the generation of mechanical force in eukaryotic cells. Muscle myosins are heterohexamers composed of 2 myosin heavy chains and 2 pairs of nonidentical myosin light chains. This gene encodes a member of the class II or conventional myosin heavy chains, and functions in skeletal muscle contraction. This gene is predominantly expressed in fetal skeletal muscle. This gene is found in a cluster of myosin heavy chain genes on chromosome 17. A mutation in this gene results in trismus-pseudocamptodactyly syndrome. [provided by RefSeq, Sep 2009]

Uniprot Description

MYH8: Muscle contraction. Defects in MYH8 are a cause of Carney complex variant (CACOV). Carney complex is a multiple neoplasia syndrome characterized by spotty skin pigmentation, cardiac and other myxomas, endocrine tumors, and psammomatous melanotic schwannomas. Familial cardiac myxomas are associated with spotty pigmentation of the skin and other phenotypes, including primary pigmented nodular adrenocortical dysplasia, extracardiac (frequently cutaneous) myxomas, schwannomas, and pituitary, thyroid, testicular, bone, ovarian, and breast tumors. Cardiac myxomas do not develop in all patients with the Carney complex, but affected patients have at least two features of the complex or one feature and a clinically significant family history. Defects in MYH8 are a cause of distal arthrogryposis type (DA7). A hereditary distal arthrogryposis characterized by an inability to open the mouth fully (trismus) and pseudocamptodactyly in which wrist dorsiflexion, but not volarflexion, produces involuntary flexion contracture of distal and proximal interphalangeal joints. Such hand and jaw contractures are caused by shortened flexor muscle-tendon units. Similar lower-limb contractures also produce foot deformity. The trismus-pseudocamptodactyly syndrome is a morbid autosomal dominant trait with variable expressivity but high penetrance. In these patients, trismus complicates dental care, feeding during infancy, and intubation for anesthesia, and the pseudocamptodactyly impairs manual dexterity, with consequent occupational and social disability. Many patients require surgical correction of contractures.

Protein type: Motility/polarity/chemotaxis; Motor

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: sarcomere; cytoplasm; muscle myosin complex; cytosol

Molecular Function: calmodulin binding; microfilament motor activity; actin filament binding; structural constituent of muscle; ATPase activity; myosin light chain binding; ATP binding

Biological Process: skeletal muscle contraction; muscle contraction; metabolic process; muscle filament sliding

Disease: Carney Complex Variant; Arthrogryposis, Distal, Type 7