Rabbit MYH Polyclonal Antibody | anti-MYH antibody

MYH (Y176) Polyclonal Antibody

Cat. Num. AAA3002744

• Gene Names: MUTYH; MYH
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: MYH; Polyclonal Antibody; MYH (Y176) Polyclonal Antibody; A/G-specific adenine DNA glycosylase; MutY homolog; hMYH; MUTYH; anti-MYH antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MYH (Y176) polyclonal antibody detects endogenous levels of MYH protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 535

• Applicable Applications for anti-MYH antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 142-190 of Human MYH.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of MYH (Y176) pAb at 1:500 dilutionLane1:K562 whole cell lysate (40ug) Lane2:U-87MG whole cell lysate (40ug) Lane3:C6 whole cell lysate (40ug) Lane4:AML-12 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of MYH (Y176) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.)

Related Product Information for anti-MYH antibody

MYH (mutY homolog (E. coli)) is a DNA glycosylase mismatch repair enzyme that in conjunction with mutM (OGG1), cleaves adenine residues paired with either oxidized (8-hydroxyguanines) or non-modified guanines in order to correct A/G and A/C mismatches. Repair of most modified and mispaired bases in the genome is initiated by DNA glycosylases, which bind and cleave N-glycosyl bonds to initiate base excision repair. MYH is crucial for the avoidance of mutations resulting from oxidative DNA damage. Multiple N-terminal splice variants of MYH exist in mammalian cells. Increasing levels of MYH in A549 cells exposed to oxygen and infrared radiation leads to improvements in cell survival. Biallelic MYH germ-line mutations predispose humans to colorectal adenomas and carcinomas. MYH is abundant in neurons where mitochondrial genomes exposed to reactive oxygen species (ROS) that damage DNA must maintain integrity over the entire mammalian life span.

NCBI and Uniprot Product Information

• NCBI GI #: 115298648
• NCBI GeneID: 4595
• NCBI Accession #: NP_001041636.1
• NCBI GenBank Nucleotide #: NP_001041636.1
• UniProt Accession #: Q9UIF7
• Molecular Weight: ~ 60kDa
• NCBI Official Full Name: adenine DNA glycosylase isoform 2
• NCBI Official Synonym Full Names: mutY DNA glycosylase
• NCBI Official Symbol: MUTYH
• NCBI Official Synonym Symbols: MYH
• NCBI Protein Information: adenine DNA glycosylase
• UniProt Protein Name: A/G-specific adenine DNA glycosylase
• UniProt Gene Name: MUTYH
• UniProt Synonym Gene Names: MYH; hMYH
• UniProt Entry Name: MUTYH_HUMAN

NCBI Description

This gene encodes a DNA glycosylase involved in oxidative DNA damage repair. The enzyme excises adenine bases from the DNA backbone at sites where adenine is inappropriately paired with guanine, cytosine, or 8-oxo-7,8-dihydroguanine, a major oxidatively damaged DNA lesion. The protein is localized to the nucleus and mitochondria. This gene product is thought to play a role in signaling apoptosis by the introduction of single-strand breaks following oxidative damage. Mutations in this gene result in heritable predisposition to colorectal cancer, termed MUTYH-associated polyposis (MAP). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2017]

Uniprot Description

MUTYH: Involved in oxidative DNA damage repair. Initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. Possesses both adenine and 2- OH-A DNA glycosylase activities. Belongs to the Nth/MutY family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA repair, damage; EC 3.2.2.-; Tumor suppressor; Hydrolase; Mitochondrial

Chromosomal Location of Human Ortholog: 1p34.1

Cellular Component: nucleoplasm; mitochondrion; nucleus

Molecular Function: protein binding; 4 iron, 4 sulfur cluster binding; metal ion binding; hydrolase activity, acting on glycosyl bonds; MutLalpha complex binding; MutSbeta complex binding; MutLbeta complex binding; MutSalpha complex binding

Biological Process: mismatch repair; depurination; base-excision repair, AP site formation; base-excision repair; DNA repair

Disease: Gastric Cancer; Pilomatrixoma; Familial Adenomatous Polyposis, 2