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Western Blot (WB) ((0.5ug/ml) staining of Muscle lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.)

Goat MYF5 Polyclonal Antibody | anti-MYF5 antibody

Goat anti-MYF5 Antibody

Gene Names
MYF5; bHLHc2
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
MYF5; Polyclonal Antibody; Goat anti-MYF5 Antibody; myogenic factor 5; MYF5 antibody; myogenic factor 5 antibody; anti-MYF5 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
PECNSPVWSRKSST
Sequence Length
255
Applicable Applications for anti-MYF5 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Immunogen
Peptide with sequence PECNSPVWSRKSST, from the internal region of the protein sequence according to NP_005584.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.5ug/ml) staining of Muscle lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((0.5ug/ml) staining of Muscle lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,296 Da
NCBI Official Full Name
myogenic factor 5
NCBI Official Synonym Full Names
myogenic factor 5
NCBI Official Symbol
MYF5
NCBI Official Synonym Symbols
bHLHc2
NCBI Protein Information
myogenic factor 5
UniProt Protein Name
Myogenic factor 5
Protein Family
UniProt Gene Name
MYF5
UniProt Synonym Gene Names
BHLHC2; Myf-5; bHLHc2
UniProt Entry Name
MYF5_HUMAN

Uniprot Description

Myf-5: Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 12q21

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; protein heterodimerization activity; RNA polymerase II transcription factor activity, enhancer binding

Biological Process: camera-type eye development; cartilage condensation; embryonic skeletal morphogenesis; extracellular matrix organization and biogenesis; muscle cell fate commitment; muscle development; ossification; positive regulation of muscle cell differentiation; positive regulation of myoblast differentiation; positive regulation of skeletal muscle fiber development; positive regulation of transcription from RNA polymerase II promoter; regulation of cell-matrix adhesion; regulation of transcription from RNA polymerase II promoter; skeletal muscle development; somitogenesis; transcription from RNA polymerase II promoter

Research Articles on MYF5

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Product Notes

The MYF5 myf5 (Catalog #AAA420709) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-MYF5 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's MYF5 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Researchers should empirically determine the suitability of the MYF5 myf5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: PECNSPVWSR KSST. It is sometimes possible for the material contained within the vial of "MYF5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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