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Western Blot (WB)
(Western Blot: Sample: Human Hela Cells.)
Rabbit anti-Mouse Myeloproliferative Leukemia Virus Oncogene (MPL) Polyclonal Antibody | anti-MPL antibody
Polyclonal Antibody to Myeloproliferative Leukemia Virus Oncogene (MPL)
Gene Names
Mpl; CD110; TPO-R; c-mpl; hlb219
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Myeloproliferative Leukemia Virus Oncogene (MPL); Polyclonal Antibody; Polyclonal Antibody to Myeloproliferative Leukemia Virus Oncogene (MPL); anti-MPL antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against MPL. It has been selected for its ability to recognize MPL in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LYAYR GEKPRACPLY SQSVPTFGTR YVCQFPAQDE VRLFFPLHLW VKNVSLNQTL IQRVLFVDSV GLPAPPRVIK ARGGSQPGEL QIHWEAPAPE ISDFLRHELR YGPTDSSNAT APSVIQLLST ETCCPTLWMP NPVPVLDQPP CVHPTASQPH GPAPFLTVKG GSCLVSGLQA GKSYWLQLRS
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LYAYR GEKPRACPLY SQSVPTFGTR YVCQFPAQDE VRLFFPLHLW VKNVSLNQTL IQRVLFVDSV GLPAPPRVIK ARGGSQPGEL QIHWEAPAPE ISDFLRHELR YGPTDSSNAT APSVIQLLST ETCCPTLWMP NPVPVLDQPP CVHPTASQPH GPAPFLTVKG GSCLVSGLQA GKSYWLQLRS
Sequence Length
628
Applicable Applications for anti-MPL antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant MPL (Leu66~Ser260) expressed in E.coli.
Cross Reactivity
Human, Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2055533
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Human Hela Cells.)
Western Blot (WB)
(Western Blot: Sample: Human Hela Cells.)
Western Blot (WB)
(Western Blot: Sample: Recombinant MPL, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant MPL, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Lung Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Lung Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Pancreas Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Pancreas Tissue.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
69,788 Da
NCBI Official Full Name
thrombopoietin receptor isoform 1
NCBI Official Synonym Full Names
myeloproliferative leukemia virus oncogene
NCBI Official Symbol
Mpl
NCBI Official Synonym Symbols
CD110; TPO-R; c-mpl; hlb219
NCBI Protein Information
thrombopoietin receptor
UniProt Protein Name
Thrombopoietin receptor
Protein Family
UniProt Gene Name
Mpl
UniProt Synonym Gene Names
Tpor; TPO-R
Uniprot Description
MPL: a hematopoietic receptor for thrombopoietin. Thrombopoietin is the major regulator of megakaryocytopoiesis and platelet formation. Dimerizes upon binding of thrombopoietin, leading to its phosphorylation and activation of JAKs and STATs. May represent a regulatory molecule specific for TPO-R-dependent immune responses. Two splice-variant isoforms have been described.
Protein type: Membrane protein, integral; Receptor, cytokine
Chromosomal Location of Human Ortholog: 4 D2.1|4 54.61 cM
Cellular Component: cell soma; cell surface; Golgi apparatus; integral component of membrane; membrane; neuron projection; plasma membrane
Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; thrombopoietin receptor activity
Biological Process: basophil homeostasis; eosinophil homeostasis; homeostasis of number of cells; negative regulation of apoptosis; neutrophil homeostasis; platelet formation; positive regulation of gene expression; positive regulation of lymphocyte proliferation; positive regulation of platelet formation; regulation of chemokine production; regulation of stem cell division; regulation of stem cell proliferation
Research Articles on MPL
Similar Products
Product Notes
The MPL mpl (Catalog #AAA2017492) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Myeloproliferative Leukemia Virus Oncogene (MPL) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Myeloproliferative Leukemia Virus Oncogene (MPL) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the MPL mpl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LYAYR GEKPRACPLY SQSVPTFGTR YVCQFPAQDE VRLFFPLHLW VKNVSLNQTL IQRVLFVDSV GLPAPPRVIK ARGGSQPGEL QIHWEAPAPE ISDFLRHELR YGPTDSSNAT APSVIQLLST ETCCPTLWMP NPVPVLDQPP CVHPTASQPH GPAPFLTVKG GSCLVSGLQA GKSYWLQLRS. It is sometimes possible for the material contained within the vial of "Myeloproliferative Leukemia Virus Oncogene (MPL), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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