Western Blot (WB)
(Western BlotSample: Recombinant MBP, Rat.)
Rabbit anti-Rat Major Basic Protein (MBP) Polyclonal Antibody | anti-MBP antibody
Polyclonal Antibody to Major Basic Protein (MBP)
Reactivity
Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Major Basic Protein (MBP); Polyclonal Antibody; Polyclonal Antibody to Major Basic Protein (MBP); anti-MBP antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
0.75mg/ml (varies by lot)
Applicable Applications for anti-MBP antibody
Western Blot (WB), Immunohistochemistry (IHC) - Formalin/Paraffin, Immunocytochemistry (ICC), Immunoprecipitation (IP).
Application Notes
Western blotting: 0.5-2ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Organism Species
Rattus norvegicus (Rat)
Source
Polyclonal antibody preparation
Cross Reactivity
Rat
Traits
Liquid
Immunogen
Recombinant MBP (Gly80~Lys219 (Accession # Q63189)) expressed in E.coli ( MBS2018803 ).
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 24 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 24 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western BlotSample: Recombinant MBP, Rat.)
Western Blot (WB)
(Western BlotSample: Recombinant MBP, Rat.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
bone marrow proteoglycan
NCBI Official Synonym Full Names
proteoglycan 2
NCBI Official Symbol
Prg2
NCBI Protein Information
bone marrow proteoglycan
UniProt Protein Name
Bone marrow proteoglycan
Protein Family
UniProt Gene Name
Prg2
UniProt Synonym Gene Names
Mbp; BMPG; EMBP; MBP
NCBI Description
human homolog is the primary component of the eosinophil granule crystalline core; may play a role in allergic response and asthma [RGD, Feb 2006]
Uniprot Description
PRG2: Cytotoxin and helminthotoxin. Also induces non-cytolytic histamine release from human basophils. Involved in antiparasitic defense mechanisms and immune hypersensitivity reactions. The proform acts as a proteinase inhibitor, reducing the activity of PAPPA.
Protein type: Secreted; Secreted, signal peptide; Vesicle
Chromosomal Location of Human Ortholog: 3q24
Molecular Function: carbohydrate binding; heparin binding
Biological Process: defense response to bacterium; defense response to nematode; immune response; negative regulation of interleukin-10 production; positive regulation of interleukin-4 production; regulation of cytokine biosynthetic process
Research Articles on MBP
Similar Products
Product Notes
The MBP prg2 (Catalog #AAA2016625) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Major Basic Protein (MBP) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Major Basic Protein (MBP) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) - Formalin/Paraffin, Immunocytochemistry (ICC), Immunoprecipitation (IP). . Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the MBP prg2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Major Basic Protein (MBP), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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