Rabbit Myeloid differentiation primary response protein MyD88 Polyclonal Antibody | anti-MYD88 antibody

Anti-MyD88 Antibody

Cat. Num. AAA177429

• Gene Names: MYD88; MYD88D
• Reactivity: Human, Mouse, Rat. No cross reactivity with other proteins.
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: Myeloid differentiation primary response protein MyD88; Polyclonal Antibody; Anti-MyD88 Antibody; MYD 88 antibody; Myd88 antibody; MYD88_HUMAN antibody; MYD88D antibody; Myeloid differentiation marker 88 antibody; Myeloid differentiation primary response gene 88 antibody; Myeloid differentiation primary response gene antibody; Myeloid differentiation primary response protein MyD88 antibody; OTTHUMP00000161718 antibody; OTTHUMP00000208595 antibody; OTTHUMP00000209058 antibody; OTTHUMP00000209059 antibody; OTTHUMP00000209060 antibody; myeloid differentiation primary response 88; anti-MYD88 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat. No cross reactivity with other proteins.
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 159

• Applicable Applications for anti-MYD88 antibody: Western Blot (WB), Immunohistochemistry (IHC-P)
• Application Notes: Western Blot: Concentration: 0.1-0.5mug/ml; Tested Species: Human, Rat; Immunohistochemistry (Paraffin-embedded Section): Concentration: 0.5-1mug/ml; Tested Species: Human, Mouse, Rat
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Immunogen: E.coli-derived human MyD88 recombinant protein (Position: A44-F264). Human MyD88 shares 84% and 83% amino acid (aa) sequences identity with mouse and rat MyD88, respectively.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Testing Data

(Anti-MyD88 Picoband antibody, MBS177429-1.jpgAll lanes: Anti MYD88 (MBS177429) at 0.5ug/mlWB: Recombinant Human MYD88 Protein 0.5ngPredicted bind size: 49KDObserved bind size: 49KD )

Testing Data

(Anti-MyD88 Picoband antibody, MBS177429-2.jpgAll lanes: Anti MYD88 (MBS177429) at 0.5ug/mlLane 1: Rat Cardiac Muscle Tissue Lysate at 50ugLane 2: HELA Whole Cell Lysate at 40ugLane 3: MCF Whole Cell Lysate at 40ugLane 4: HEPG2 Whole Cell Lysate at 40ugLane 5: JURKAT Whole Cell Lysate at 40ugLane 6: RAJI Whole Cell Lysate at 40ugPredicted bind size: 33KDObserved bind size: 33KD )

Immunohistochemistry (IHC)

(Anti-MyD88 Picoband antibody, MBS177429-3.JPGIHC(P): Rat Lung Tissue )

Immunohistochemistry (IHC)

(Anti-MyD88 Picoband antibody, MBS177429-4.JPGIHC(P): Human Intestinal Cancer Tissue )

Immunohistochemistry (IHC)

(Anti-MyD88 Picoband antibody, MBS177429-5.JPGIHC(P): Mouse Spleen Tissue )

Related Product Information for anti-MYD88 antibody

Description: Rabbit IgG polyclonal antibody for Myeloid differentiation primary response protein MyD88(MYD88) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Background: MYD88(MYELOID DIFFERENTIATION PRIMARY RESPONSE GENE 88), is a protein that, in humans, is encoded by the MYD88 gene. MyD88 is a key downstream adapter for most Toll-like receptors (TLRs) and interleukin-1 receptors (IL1Rs). And it is mapped on 3p22.2. MYD88 encodes a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. This protein functions as an essential signal transducer in the interleukin-1 and Toll-like receptor signaling pathways. Overexpression of MYD88 caused an increase in the level of transcription from the interleukin-8 promoter. The C-terminal domain of MYD88 has significant sequence similarity to the cytoplasmic domain of IL1RAP. Inhibiting the IL1R-MYD88 pathway in vivo could block the damage from acute inflammation that occurs in response to sterile cell death, and do so in a way that might not compromise tissue repair or host defense against pathogens.

NCBI and Uniprot Product Information

• NCBI GI #: 289546500
• NCBI GeneID: 4615
• NCBI Accession #: NP_001166037.1
• NCBI GenBank Nucleotide #: NM_001172566.1
• UniProt Accession #: Q99836; P78397; Q53XS7; B4DKH8; B4DKU4; B4DQ60; B4DQ72; J3KPU4; J3KQ87; J3KQJ6
• Molecular Weight: 34,097 Da
• NCBI Official Full Name: myeloid differentiation primary response protein MyD88 isoform 5
• NCBI Official Synonym Full Names: myeloid differentiation primary response 88
• NCBI Official Symbol: MYD88
• NCBI Official Synonym Symbols: MYD88D
• NCBI Protein Information: myeloid differentiation primary response protein MyD88; myeloid differentiation primary response gene (88)
• UniProt Protein Name: Myeloid differentiation primary response protein MyD88
• Protein Family: Myeloid differentiation primary response protein
• UniProt Gene Name: MYD88
• UniProt Entry Name: MYD88_HUMAN

NCBI Description

This gene encodes a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. This protein functions as an essential signal transducer in the interleukin-1 and Toll-like receptor signaling pathways. These pathways regulate that activation of numerous proinflammatory genes. The encoded protein consists of an N-terminal death domain and a C-terminal Toll-interleukin1 receptor domain. Patients with defects in this gene have an increased susceptibility to pyogenic bacterial infections. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]

Uniprot Description

MYD88: Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. Homodimer. Also forms heterodimers with TIRAP. Binds to TLR2, TLR4, IRAK1, IRAK2 and IRAK4 via their respective TIR domains. Interacts with IL18R1. Interacts with BMX, IL1RL1 and IRF7. Interacts with LRRFIP1 and LRRFIP2; this interaction positively regulates Toll-like receptor (TLR) signaling in response to agonist. Interacts with FLII. LRRFIP1 and LRRFIP2 compete with FLII for MYD88-binding. Interacts with IRF1. Ubiquitous. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 3p22

Cellular Component: plasma membrane; endosome membrane; cytosol

Molecular Function: identical protein binding; protein binding; death receptor binding

Biological Process: positive regulation of interleukin-17 production; positive regulation of I-kappaB kinase/NF-kappaB cascade; nerve growth factor receptor signaling pathway; positive regulation of interleukin-23 production; positive regulation of interleukin-6 production; signal transduction; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; defense response to Gram-positive bacterium; cell surface receptor linked signal transduction; regulation of inflammatory response; positive regulation of interferon type I production; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; inflammatory response; toll-like receptor 4 signaling pathway; negative regulation of apoptosis

Disease: Macroglobulinemia, Waldenstrom, Susceptibility To, 1; Myd88 Deficiency