Chicken Myelin Basic Protein (MBP) Polyclonal Antibody | anti-MBP antibody

Anti-Myelin Basic Protein (MBP)

Cat. Num. AAA502161

• Gene Names: Mbp; Mbps
• Reactivity: Bovine, human, mouse, rat
• Applications: Western Blot, Immunofluorescence
• Purity: Total IgY fraction (Total IgY fraction.)
• Synonyms: Myelin Basic Protein (MBP); Polyclonal Antibody; Anti-Myelin Basic Protein (MBP); anti-MBP antibody

• Host: Chicken
• Reactivity: Bovine, human, mouse, rat
• Clonality: Polyclonal
• Isotype: IgG1
• Clone Number: 2103
• Specificity: Specific for the ~18k & 22k myelin basic protein in Western blots of rat brain homogenate.
• Purity/Purification: Total IgY fraction (Total IgY fraction.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 195

• Applicable Applications for anti-MBP antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:5,000; IF: 1:500
• Antigen: Three peptide sequences conserved in higher vertebrate MBP proteins.
• Immunogen Information: Three peptide sequences conserved in higher vertebrate MBP proteins
• Immunogen Species: Rat
• Reactivity Assumed Based on 100% Sequence Homology: Most mammals
• Species Reactivity Note: The antibody has been directly tested for reactivity in bovine, human, mouse and rat. It is expected that the antibody will react in other mammalian tissues.
• Biological Significance: Myelin Basic Protein (MBP) is one of the major proteins of the myelin sheath surrounding axons in the nervous system. Since it is of relatively low molecular weight and high abundance the protein sequence was determined from purified protein over 30 years ago (Eylar et al., 1971). The protein is made by oligodendrocytes in the central nervous system, so antibodies to MBP are good markers of this cell type. However, transcripts from the same gene are also expressed in certain hematopoetic lineage cells (Marty et al., 2002). In the central nervous system there are there different forms of the protein made by alternate transcription from a single gene, which have molecular weights of 21.5, 18.5, and 17.2 kDa. Since the two lower molecular weight forms are very close in molecular size, MBP antibodies typically show two bands on Western blots, one at about 22kDa and another at about 18kDa.
• Preparation and Storage: Store at -20 degree C; stable for at least 1 year.

Testing Data

Related Product Information for anti-MBP antibody

Chicken polyclonal antibody

References

• Eylar EH, Brostoff S, Hashim G, Caccam J, Burnett P. (1971) Basic A1 protein of the myelin membrane: the complete amino acid sequence. J. Biol. Chem. 246:5770-5784.
• Marty MC, Alliot F, Rutin J, Fritz R, Trisler D. and Pessac B. (2002) The myelin basic protein gene is expressed in differentiated blood cell lineages and in hematopoietic progenitors. Proc. Nat. Acad. Sci. 99:8856-8861.

NCBI and Uniprot Product Information

• NCBI GI #: 70166245
• NCBI GeneID: 24547
• NCBI Accession #: NP_001020462.1
• NCBI GenBank Nucleotide #: NM_001025291.1
• UniProt Accession #: P02688; Q505J1; Q8R4K6; Q9Z1J4; Q9Z1J5; Q9Z1J6
• Molecular Weight: 18/22
• NCBI Official Full Name: myelin basic protein isoform 1
• NCBI Official Synonym Full Names: myelin basic protein
• NCBI Official Symbol: Mbp
• NCBI Official Synonym Symbols: Mbps
• NCBI Protein Information: Golli-Mbp; myelin basic protein; MBP S; myelin basic protein S; myelin basic protein Golli-mbp; Golli-Mbp; myelin basic protein; myelin basic protein S
• UniProt Protein Name: Myelin basic protein
• Protein Family: Myelin basic protein
• UniProt Gene Name: Mbp
• UniProt Synonym Gene Names: MBP
• UniProt Entry Name: MBP_RAT

NCBI Description

The protein encoded by the classic Mbp gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, Mbp-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long Mbp gene (otherwise called "Golli-Mbp") that contains 3 additional exons located upstream of the classic Mbp exons. Alternative splicing from the Golli and the Mbp transcription start sites gives rise to 2 sets of Mbp-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-Mbp, spliced in-frame to 1 or more Mbp exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to Mbp aa sequence. The second family of transcripts contain only Mbp exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the Mbp transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. Mutation of the Mbp gene is associated with the 'shiverer' and 'myelin deficient' phenotypes in mouse. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Is, with PLP, the most abundant protein component of the myelin membrane in the CNS. Has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. Each splice variant and charge isomer may have a specialized function in the assembly of an optimized, biochemically functional myelin membrane

By similarity.

Subunit structure: Homodimer

By similarity.

Subcellular location: Myelin membrane; Peripheral membrane protein; Cytoplasmic side. Note: Cytoplasmic side of myelin.

Tissue specificity: Found in both the central and the peripheral nervous system.

Post-translational modification: As in other animals, several charge isomers may be produced as a result of optional post-translatonial modifications, such as phosphorylation of serine or threonine residues, deamidation of glutamine or asparagine residues, citrullination and methylation of arginine residues.Arg-131 was found to be 44% monomethylated and 11% symmetrically dimethylated. Ref.6Phosphorylated by TAOK2, VRK2, MAPK11, MAPK12, MAPK14 and MINK1

By similarity. Ref.10

Sequence similarities: Belongs to the myelin basic protein family.