Rabbit MXI1 Polyclonal Antibody | anti-MXI1 antibody

MXI1 Polyclonal Antibody

Cat. Num. AAA3011655

• Gene Names: MXI1; MXI; MAD2; MXD2; bHLHc11
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: MXI1; Polyclonal Antibody; MXI1 Polyclonal Antibody; Max-interacting protein 1; Max interactor 1; Class C basic helix-loop-helix protein 11; bHLHc11; BHLHC11; anti-MXI1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MXI1 polyclonal antibody detects endogenous levels of MXI1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 182

• Applicable Applications for anti-MXI1 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IF: 1:50-1:200
• Immunogen: Recombinant full length Human MXI1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using MXI1 antibody. Blue: DAPI for nuclear staining.)

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using MXI1 antibody.)

Related Product Information for anti-MXI1 antibody

It is now well established that Myc regulation of cell proliferation and differentiation involves a family of related transcription factors. One such factor, Max, is an obligate heterodimeric partner for Myc and can also form heterodimers with at least four related proteins designated Mad 1, Mxi1, Mad 3 and Mad 4. Like Mad 1 and Mxi1, association of Mad 3 and Mad 4 with Max results in transcriptional repression. Both Myc and the Mad proteins have short half-lives and their synthesis is tightly regulated, while Max expression is constitutive and relatively stable. Two related mammalian cDNAs have been identified and shown to encode Mad-binding proteins. Both possess sequence homology with the yeast transcription repressor Sin3 including four conserved paired amphipathic helix (PAH) domains. mSin3A and mSin3B specifically interact with the Mad proteins via their second paired amphipathic helix domain (PAH2). It has been suggested that Mad-Max heterodimers repress transcription by tethering mSin3 to DNA as corepressors.

NCBI and Uniprot Product Information

• NCBI GI #: 57242784
• NCBI GeneID: 4601
• NCBI Accession #: NP_001008541.1
• NCBI GenBank Nucleotide #: NP_001008541.1
• UniProt Accession #: P50539
• Molecular Weight: ~ 35kDa
• NCBI Official Full Name: max-interacting protein 1 isoform c
• NCBI Official Synonym Full Names: MAX interactor 1, dimerization protein
• NCBI Official Symbol: MXI1
• NCBI Official Synonym Symbols: MXI; MAD2; MXD2; bHLHc11
• NCBI Protein Information: max-interacting protein 1
• UniProt Protein Name: Max-interacting protein 1
• Protein Family: Max-interacting protein
• UniProt Gene Name: MXI1
• UniProt Synonym Gene Names: BHLHC11; Max interactor 1; bHLHc11
• UniProt Entry Name: MXI1_HUMAN

NCBI Description

Expression of the c-myc gene, which produces an oncogenic transcription factor, is tightly regulated in normal cells but is frequently deregulated in human cancers. The protein encoded by this gene is a transcriptional repressor thought to negatively regulate MYC function, and is therefore a potential tumor suppressor. This protein inhibits the transcriptional activity of MYC by competing for MAX, another basic helix-loop-helix protein that binds to MYC and is required for its function. Defects in this gene are frequently found in patients with prostate tumors. Three alternatively spliced transcripts encoding different isoforms have been described. Additional alternatively spliced transcripts may exist but the products of these transcripts have not been verified experimentally. [provided by RefSeq, Jul 2008]