Rabbit anti-Human MXI1 Polyclonal Antibody | anti-MXI1 antibody

Anti-MXI1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8108638

• Reactivity: Human
• Applications: Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: MXI1; Polyclonal Antibody; Anti-MXI1 Antibody; Rabbit Polyclonal; MXI1 Antibody; Rabbit PAb; Antigen Affinity Purified; MAX interactor 1; dimerization protein; Anti-bHLHc11 Antibody; Anti-MAD2 Antibody; Anti-MXD2 Antibody; Anti-MXI Antibody; Anti-RP11-549L6.1 Antibody; anti-MXI1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human MXI1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-MXI1 antibody: Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: IHC-P: 1:50-1:200; ICC/IF: 1:100-1:500
• Immunogen: E Coli-derived Human MXI1 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human MXI1 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human MXI1 in human colon with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human MXI1 in human testis with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Immunofluorescence (IF)

(Immunofluorescence staining of MXI1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human MXI1 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)

Related Product Information for anti-MXI1 antibody

MAX interactor 1 (Mxi1) proteins are c-Myc antagonists that primarily exert their biological functions by inhibiting Myc-dependent gene transcription. Mxi1 belongs to the Myc/Max/Mad network of proteins that have been implicated in the control of multiple aspects of cellular behavior. The Mxi1 protein functions in a regulatory network with members of the c-Myc family, in which c-Myc activates transcription and stimulates cell proliferation, and Mxi1 negatively regulates these actions. Inactivation of the MXI1 gene could, therefore, inhibit differentiation and enhance proliferation in the presence of normal levels of c-Myc, and thus MXI1 is a potential tumor suppressor gene. Max interactor-1 (MXI1), an antagonist of c-Myc that is involved in brain tumor progression, has been reported to be deregulated in a variety of tumors including glioma. Mxi1 is a transcriptional repressor and inhibits the proliferation of NB cells, suggesting that Mxi1 functions as an N-Myc antagonist.

References

Zhou J, et al. (2013) Microrna-155 promotes glioma cell proliferation via the regulation of mxi1. PLoS One 8 (12): e83055.Wu L, et al. (2015) Cxcl10 expression induced by mxi1 inactivation induces mesangial cell apoptosis in mouse habu nephritis. Cell Signal 27 (5): 943-950.Wechsler DS, et al. (1997) Mxi1, a putative tumor suppressor gene, suppresses growth of human glioblastoma cells. Cancer Res 57 (21): 4905-4912.