Rabbit MVP Polyclonal Antibody | anti-MVP antibody

MVP Antibody

Cat. Num. AAA9607388

• Gene Names: MVP; LRP; VAULT1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: MVP; Polyclonal Antibody; MVP Antibody; LRP; Lung resistance related protein; Lung resistance-related protein; Major vault protein; rat; homolog of; MVP_HUMAN; testicular secretory protein Li 30; VAULT 1; VAULT1; anti-MVP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MVP antibody detects endogenous levels of total MVP
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 833

• Applicable Applications for anti-MVP antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: A synthesized peptide derived from human MVP
• Subcellular Location: Cytoplasm. Nucleus > Nuclear Pore Complex. 5% found in the nuclear pore complex. Translocates from the nucleus to the cytoplasm upon EGF treatment.
• Tissue Specificity: Present in most normal tissues. Higher expression observed in epithelial cells with secretory and excretory functions, as well as in cells chronically exposed to xenobiotics, such as bronchial cells and cells lining the intestine. Overexpressed in many multidrug-resistant cancer cells.
• Predicted Cross Reactivity: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
• Similarity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (92%), Chicken (83%), Xenopus (92%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Immunohistochemistry (IHC)

(MBS9607388 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Western Blot (WB)

(Western blot analysis of Hela whole cell lysates, using MVP Antibody. The lane on the left is treated with the antigen-specific peptide.)

Related Product Information for anti-MVP antibody

Description: This gene encodes the major component of the vault complex. Vaults are multi-subunit ribonucleoprotein structures that may be involved in nucleo-cytoplasmic transport. The encoded protein may play a role in multiple cellular processes by regulating the MAP kinase, JAK/STAT and phosphoinositide 3-kinase/Akt signaling pathways. The encoded protein also plays a role in multidrug resistance, and expression of this gene may be a prognostic marker for several types of cancer. Alternatively spliced transcript variants have been observed for this gene.
Function: Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates IFNG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases.
Subunit Structure: The vault ribonucleoprotein particle is a huge (400 A x 670 A) cage structure of 12.9 MDa. It consists of a dimer of half-vaults, with each half-vault comprising 39 identical major vault protein (MVP) chains, PARP4 and one or more vault RNAs (vRNAs). Interacts with TEP1. Interacts with PTEN and activated MAPK1. The phosphorylated protein interacts with the SH2 domains of PTPN11 and SRC. Interacts with APEX1. May interact with ZNF540.
Post-translational Modifications: Phosphorylated on Tyr residues after EGF stimulation. Dephosphorylated by PTPN11.
Similarity: MVP 3 mediates interaction with PTEN.MVP 4 mediates interaction with PARP4.

References

Zhou H, Lin C, Zhang Y, Zhang X, Zhang C, Zhang P, Xie X, Ren Z; Journal: Cell Prolif. miR-506 enhances the sensitivity of human colorectal cancer cells to oxaliplatin by suppressing MDR1/P-gp expression.

NCBI and Uniprot Product Information

• NCBI GI #: 645912958
• NCBI GeneID: 9961
• NCBI Accession #: NP_001280133.1
• NCBI GenBank Nucleotide #: NM_001293204.1
• UniProt Accession #: Q14764; Q96BG4; Q9BPW6; Q9BQT1; Q9UBD1
• Molecular Weight: Observed: 99 kDa; Predicted: 100 kDa
• NCBI Official Full Name: major vault protein isoform 2
• NCBI Official Synonym Full Names: major vault protein
• NCBI Official Symbol: MVP
• NCBI Official Synonym Symbols: LRP; VAULT1
• NCBI Protein Information: major vault protein
• UniProt Protein Name: Major vault protein
• Protein Family: Hyperpolarization-activated voltage-gated potassium channel
• UniProt Gene Name: MVP
• UniProt Synonym Gene Names: LRP; MVP

NCBI Description

This gene encodes the major component of the vault complex. Vaults are multi-subunit ribonucleoprotein structures that may be involved in nucleo-cytoplasmic transport. The encoded protein may play a role in multiple cellular processes by regulating the MAP kinase, JAK/STAT and phosphoinositide 3-kinase/Akt signaling pathways. The encoded protein also plays a role in multidrug resistance, and expression of this gene may be a prognostic marker for several types of cancer. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]

Uniprot Description

Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates IFNG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases.