Rabbit Muscarinic Receptor M5 Polyclonal Antibody | anti-CHRM5 antibody

Muscarinic Receptor M5 (Cholinergic Muscarinic Receptor M5, Chrm5)

Cat. Num. AAA615612

• Gene Names: CHRM5; HM5; MGC41838
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: Highly Purified; Purified by precipitation, dialysis and chromatography.
• Synonyms: Muscarinic Receptor M5; Polyclonal Antibody; Muscarinic Receptor M5 (Cholinergic Muscarinic Receptor M5; Chrm5); Anti -Muscarinic Receptor M5 (Cholinergic Muscarinic Receptor M5; anti-CHRM5 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes M5 Muscarinic Receptor. Reactivity
• Purity/Purification: Highly Purified; Purified by precipitation, dialysis and chromatography.
• Form/Format: Supplied as a liquid in PBS containing 10mg/ml BSA.

• Applicable Applications for anti-CHRM5 antibody: ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunocytochemistry.; Dilution: Immunocytochemistry: 0.1mg/ml using PLP-fixed cultured cells known to express the receptor.; Wester Blot: 0.05mg/ml using whole rat brain homogenate and cultured cells known to express the receptor
• Immunogen: Peptide analog of the carboxyl terminal of the M5 receptor covalently attached onto a carrier protein.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for anti-CHRM5 antibody

Antibodies; Abs to Receptors; Cholinergic

NCBI and Uniprot Product Information

• NCBI GI #: 7108336
• NCBI GeneID: 1133
• NCBI Accession #: NP_036257.1
• NCBI GenBank Nucleotide #: NM_012125.3
• UniProt Accession #: P08912; Q96RG7; Q6NUM3; Q8IVW0
• Molecular Weight: 60,074 Da
• NCBI Official Full Name: muscarinic acetylcholine receptor M5
• NCBI Official Synonym Full Names: cholinergic receptor, muscarinic 5
• NCBI Official Symbol: CHRM5
• NCBI Official Synonym Symbols: HM5; MGC41838
• NCBI Protein Information: muscarinic acetylcholine receptor M5; OTTHUMP00000159731
• UniProt Protein Name: Muscarinic acetylcholine receptor M5
• Protein Family: Muscarinic acetylcholine receptor
• UniProt Gene Name: CHRM5
• UniProt Entry Name: ACM5_HUMAN

NCBI Description

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The clinical implications of this receptor are unknown; however, stimulation of this receptor is known to increase cyclic AMP levels. [provided by RefSeq]

Uniprot Description

mAChR M5: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM5 sub-subfamily.

Protein type: GPCR, family 1; Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 15q26

Cellular Component: postsynaptic membrane; integral to plasma membrane; plasma membrane; cell junction

Molecular Function: phosphoinositide phospholipase C activity; G-protein coupled acetylcholine receptor activity

Biological Process: dopamine transport; cell proliferation; gastric acid secretion; acetylcholine receptor signaling, muscarinic pathway; metabolic process; muscarinic acetylcholine receptor, adenylate cyclase inhibiting pathway; transmission of nerve impulse