Rat Mitochondrial uncoupling protein 3 ELISA Kit | Ucp3 elisa kit

Rat Mitochondrial uncoupling protein 3 ELISA Kit

Cat. Num. AAA762875

• Gene Names: UCP3; SLC25A9
• Reactivity: Rat
• Synonyms: Mitochondrial uncoupling protein 3; Rat Mitochondrial uncoupling protein 3 ELISA Kit; Ucp3; Ucp3 elisa kit

• Reactivity: Rat
• Sequence Length: 312

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Ucp3 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat Ucp3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Ucp3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Ucp3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat Ucp3 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 2183021
• NCBI GeneID: 7352
• Molecular Weight: 22,874 Da
• NCBI Official Full Name: UCP3
• NCBI Official Synonym Full Names: uncoupling protein 3 (mitochondrial, proton carrier)
• NCBI Official Symbol: UCP3
• NCBI Official Synonym Symbols: SLC25A9
• NCBI Protein Information: mitochondrial uncoupling protein 3
• UniProt Protein Name: Mitochondrial uncoupling protein 3
• Protein Family: Mitochondrial uncoupling protein
• UniProt Gene Name: UCP3
• UniProt Synonym Gene Names: SLC25A9; UCP 3
• UniProt Entry Name: UCP3_HUMAN

NCBI Description

Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. The different UCPs have tissue-specific expression; this gene is primarily expressed in skeletal muscle. This gene's protein product is postulated to protect mitochondria against lipid-induced oxidative stress. Expression levels of this gene increase when fatty acid supplies to mitochondria exceed their oxidation capacity and the protein enables the export of fatty acids from mitochondria. UCPs contain the three solcar protein domains typically found in MACPs. Two splice variants have been found for this gene.[provided by RefSeq, Nov 2008]

Uniprot Description

UCP3: UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation. As a result, energy is dissipated in the form of heat. May play a role in the modulation of tissue respiratory control. Participates in thermogenesis and energy balance. Defects in UCP3 may be involved in obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Belongs to the mitochondrial carrier family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Membrane protein, multi-pass; Membrane protein, integral; Transporter

Chromosomal Location of Human Ortholog: 11q13.4

Cellular Component: integral to membrane; mitochondrial inner membrane; mitochondrion

Molecular Function: oxidative phosphorylation uncoupler activity; protein binding; structural constituent of ribosome; transporter activity

Biological Process: aging; cellular metabolic process; cellular response to hormone stimulus; fatty acid metabolic process; lipid metabolic process; mitochondrial transport; proton transport; respiratory gaseous exchange; response to activity; response to cold; response to glucocorticoid stimulus; response to hypoxia; response to insulin stimulus; response to nutrient; response to superoxide; translation

Disease: Obesity