Rabbit anti-Rat Muscarinic Acetylcholine Receptor (M3) Polyclonal Antibody | anti-CHRM3 antibody

Anti-Muscarinic Acetylcholine Receptor (M3) Antibody

Cat. Num. AAA4157637

• Gene Names: CHRM3; HM3; PBS; EGBRS
• Reactivity: Rat
• Applications: Western Blot
• Purity: Affinity Purified On Immobilized Antigen.
• Synonyms: Muscarinic Acetylcholine Receptor (M3); Polyclonal Antibody; Anti-Muscarinic Acetylcholine Receptor (M3) Antibody; anti-CHRM3 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purified On Immobilized Antigen.
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)
• Sequence Length: 590

• Applicable Applications for anti-CHRM3 antibody: Western Blot (WB)
• Immunogen: Peptide, (C)TLAKRFALKTRSQITKRKR corresponding to residues 461-479 of rat m3. (Accession number P08483).
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Related Product Information for anti-CHRM3 antibody

The action of the neurotransmitter acetylcholine is mediated through two types of receptors, the ionotrophic nicotinic receptors and the metabotrophic muscarinic receptors. The muscarinic receptors belong to the superfamily of 7-TM G-protein-coupled receptors. Five subtypes of muscarinic receptors have been cloned and named m1-m5.1-2 The muscarinic receptors are widely distributed throughout the body, but are predominantly expressed within the parasympathetic nervous system and exerts both excitatory and inhibitory control over central and peripheral tissues.1-2 The m3 receptor is expressed in brain, heart, urinary bladder, salivary gland and airway smooth muscle.3 Muscarinic receptors participate in a number of physiologic functions such as regulation of heart rate, muscle contraction, cognition, sensory processing and motor control.1 They also participate in learning and memory processing.4-5 The m1 receptors are the most abundant muscarinic subtype in the cortex and striatum. m1 receptors were also localized in the myenteric plexus where they function as autoreceptors to enhance the release of Ach from the nerves.6-7 m2, found in heart, is coupled to and mediates the slowing of heart rate in response to acetylcholine.3, 8 m3 controls smooth muscle contraction and its stimulation causes secretion from glandular tissues.9 Involvement of muscarinic receptors in apoptosis and cell growth has been reported.10-14 m1, m3 and m5, which are coupled to Gq/11 proteins, can protect cells from undergoing apoptosis induced by DNA damage. The signaling mechanism that mediates this anti-apoptotic response is still poorly understood. However, it was recently reported that a poly-basic motif in the C-terminus tail of the m3, m1 and m5 receptors is an essential element for the anti-apoptotic response of those receptors.10 Muscarinic receptors are also capable of promoting cell proliferation in many cell types, however, the signaling pathways are yet, unclear.11-14 In the literature, m3 was reported to be involved in mitogenesis and proliferation of different cell types. Recently, it was reported that activation of the m3 muscarinic receptor induces significant proliferation of colon and prostate cancer cells.11-14

NCBI and Uniprot Product Information

• NCBI GI #: 1113820506
• NCBI GeneID: 1131
• NCBI GenBank Nucleotide #: NP_001334645.1
• UniProt Accession #: P20309
• NCBI Official Full Name: muscarinic acetylcholine receptor M3
• NCBI Official Synonym Full Names: cholinergic receptor muscarinic 3
• NCBI Official Symbol: CHRM3
• NCBI Official Synonym Symbols: HM3; PBS; EGBRS
• NCBI Protein Information: muscarinic acetylcholine receptor M3
• UniProt Protein Name: Muscarinic acetylcholine receptor M3
• Protein Family: Muscarinic acetylcholine receptor
• UniProt Gene Name: CHRM3
• UniProt Entry Name: ACM3_HUMAN

NCBI Description

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 3 controls smooth muscle contraction and its stimulation causes secretion of glandular tissue. Alternative promoter use and alternative splicing results in multiple transcript variants that have different tissue specificities. [provided by RefSeq, Dec 2016]

Uniprot Description

mAChR M3: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. Defects in CHRM3 are the cause of Eagle-Barrett syndrome (EGBRS). EGBRS is a syndrome characterized by thin abdominal musculature with overlying lax skin, cryptorchism, megacystis with disorganized detrusor muscle, and urinary tract abnormalities. Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM3 sub-subfamily.

Protein type: GPCR, family 1; Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 1q43

Cellular Component: asymmetric synapse; postsynaptic membrane; integral to plasma membrane; basolateral plasma membrane; dendrite; plasma membrane; nerve terminal; cell junction

Molecular Function: protein binding; receptor activity; drug binding; acetylcholine binding; phosphoinositide phospholipase C activity; G-protein coupled acetylcholine receptor activity

Biological Process: nervous system development; cell proliferation; G-protein coupled receptor protein signaling pathway; smooth muscle contraction; acetylcholine receptor signaling, muscarinic pathway; regulation of vascular smooth muscle contraction; energy reserve metabolic process; protein modification process; positive regulation of smooth muscle contraction; signal transduction; regulation of insulin secretion; saliva secretion

Disease: Abdominal Muscles, Absence Of, With Urinary Tract Abnormality And Cryptorchidism