Goat anti-Mouse MUCDHL Polyclonal Antibody | anti-CDHR5 antibody
MUCDHL (Mucin and Cadherin-Like Protein, mu-Protocadherin)
Gene Names
CDHR5; MUCDHL; MUPCDH; MU-PCDH; FLJ20219; CDHR5
Reactivity
Mouse
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
MUCDHL; Polyclonal Antibody; MUCDHL (Mucin and Cadherin-Like Protein; mu-Protocadherin); Anti -MUCDHL (Mucin and Cadherin-Like Protein; anti-CDHR5 antibody
Host
Goat
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse MUCDHL. In direct ELISAs and Western Blots, this antibody shows approximately 5% crossreactivity with rhMUCDHL.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder in PBS with 5% trehalose.
Applicable Applications for anti-CDHR5 antibody
ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Western Blot, Flow Cytometry and Direct ELISA.
Dilution: Western Blot: Can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse MUCDHL. The detection limit for rmMUCDHL is approximately 1 ng/lane under non-reducing and reducing conditions.
Flow Cytometry: Tested in flow cytometry using primary kidney cells. Dilute this antibody to 25ug/ml and add 10 L of the diluted solution to 1-2.5 x 10e5 cells in a total reaction volume not exceeding 200 L. The binding of unlabeled antibodies may be visualized by adding a secondary developing reagent such as anti-goat IgG conjugated to a fluorochrome.
Direct ELISA: Can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect mouse MUCDHL. The detection limit for rmMUCDHL is approximately 0.5 ng/well.
Dilution: Western Blot: Can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse MUCDHL. The detection limit for rmMUCDHL is approximately 1 ng/lane under non-reducing and reducing conditions.
Flow Cytometry: Tested in flow cytometry using primary kidney cells. Dilute this antibody to 25ug/ml and add 10 L of the diluted solution to 1-2.5 x 10e5 cells in a total reaction volume not exceeding 200 L. The binding of unlabeled antibodies may be visualized by adding a secondary developing reagent such as anti-goat IgG conjugated to a fluorochrome.
Direct ELISA: Can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect mouse MUCDHL. The detection limit for rmMUCDHL is approximately 0.5 ng/well.
Immunogen
Purified, NS0-derived rmMUCDHL (rmMUCDHL; aa 29 - 479; Accession # NP_082345)
Reconstitution
Reconstitute with sterile PBS. If 0.5ml of PBS is used, the antibody concentration will be 0.2mg/ml.
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-CDHR5 antibody
Mucin and cadherin-like protein (MUCDHL), also known as mu-protocadherin, is a 100kD type I transmembrane protein belonging to the protocadherin family of cell adhesion molecules. It is found apically on the brush border of renal proximal tubule epithelial cells. The extracellular domain of mature mouse MUCDHL contains four cadherin domains plus three repeat regions that constitute a mucin stalk (aa 541-614). At least one splice variant exists in mouse that shows a deletion of aa's 459-620. Over aa's 29-479, mouse MUCDHL shares 63% and 79% aa identity with human and rat MUCDHL, respectively.
Product Categories/Family for anti-CDHR5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
69,610 Da
NCBI Official Full Name
MUCDHL
NCBI Official Synonym Full Names
cadherin-related family member 5
NCBI Official Symbol
CDHR5
NCBI Official Synonym Symbols
MUCDHL; MUPCDH; MU-PCDH; FLJ20219; CDHR5
NCBI Protein Information
cadherin-related family member 5; mu-protocadherin; OTTHUMP00000162884; mucin and cadherin-like protein
UniProt Protein Name
Mucin-like protocadherin
Protein Family
UniProt Gene Name
MUPCDH
UniProt Entry Name
Q58EZ6_HUMAN
NCBI Description
This gene is a novel mucin-like gene that is a member of the cadherin superfamily. While encoding nonpolymorphic tandem repeats rich in proline, serine and threonine similar to mucin proteins, the gene also contains sequence encoding calcium-binding motifs found in all cadherins. The role of the hybrid extracellular region and the specific function of this protein have not yet been determined. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]
Uniprot Description
MUCDHL: Acts as a calcium-dependent cell adhesion protein. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 11p15.5
Cellular Component: plasma membrane; integral to membrane
Molecular Function: protein binding; beta-catenin binding; calcium ion binding
Biological Process: homophilic cell adhesion; cell adhesion
Research Articles on CDHR5
1. MUCDHL genes are biallelically expressed in multiple fetal and adult tissues both in humans and in mice
2. identifed the human mu-protocadherin ortholog and examined its expression in the developing kidney
2. identifed the human mu-protocadherin ortholog and examined its expression in the developing kidney
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Product Notes
The CDHR5 mupcdh (Catalog #AAA617321) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The MUCDHL (Mucin and Cadherin-Like Protein, mu-Protocadherin) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MUCDHL can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Flow Cytometry and Direct ELISA. Dilution: Western Blot: Can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse MUCDHL. The detection limit for rmMUCDHL is approximately 1 ng/lane under non-reducing and reducing conditions. Flow Cytometry: Tested in flow cytometry using primary kidney cells. Dilute this antibody to 25ug/ml and add 10 L of the diluted solution to 1-2.5 x 10e5 cells in a total reaction volume not exceeding 200 L. The binding of unlabeled antibodies may be visualized by adding a secondary developing reagent such as anti-goat IgG conjugated to a fluorochrome. Direct ELISA: Can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect mouse MUCDHL. The detection limit for rmMUCDHL is approximately 0.5 ng/well. Researchers should empirically determine the suitability of the CDHR5 mupcdh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MUCDHL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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