Immunofluorescence (IF)
((4ug/ml) staining of Mouse SubMandibular Gland cells at E18. Nuclear counterstain with DAPI in green.)
Goat Muc19/Smgc Polyclonal Antibody | anti-Smgc antibody
Goat anti-Muc19/Smgc Antibody
Gene Names
Smgc; Muc19; Sfc21; DXImx49e; Muc19/Smgc; 2310010P21Rik
Reactivity
Tested: Mouse; Expected from sequence similarity: Mouse
Applications
ELISA, Immunocytochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Muc19/Smgc; Polyclonal Antibody; Goat anti-Muc19/Smgc Antibody; Smgc; submandibular gland protein C; 2310010P21Rik; DXImx49e; Muc19; Sfc21; OTTMUSP00000022832; neonatal submandibular gland protein C; Smgc antibody; submandibular gland protein C antibody; 2310010P21Rik antibody; DXImx49e antibody; Muc19 antibody; Sfc21 antibody; OTTMUSP00000022832 antibody; neonatal submandibular gland protein C antibody; Muc19 / Smgc; anti-Smgc antibody
Host
Goat
Reactivity
Tested: Mouse; Expected from sequence similarity: Mouse
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
KLEPKYENPTNGS
Sequence Length
733
Applicable Applications for anti-Smgc antibody
Peptide ELISA (EIA), Immunocytochemistry (ICC)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Preliminary experiments gave bands at approx 65kDa and 70kDa in Mouse fetal Lung lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 74.4kDa according to NP_945121.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Immunocytochemistry: Positive staining of select epithelial cells in the murine Submanibular gland at E18. Recommended concentration 4ug/ml.
Western Blot: Preliminary experiments gave bands at approx 65kDa and 70kDa in Mouse fetal Lung lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 74.4kDa according to NP_945121.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Immunocytochemistry: Positive staining of select epithelial cells in the murine Submanibular gland at E18. Recommended concentration 4ug/ml.
Immunogen
Peptide with sequence C-KLEPKYENPTNGS, from the internal region of the protein sequence according to NP_945121.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Immunofluorescence (IF)
((4ug/ml) staining of Mouse SubMandibular Gland cells at E18. Nuclear counterstain with DAPI in green.)
Immunofluorescence (IF)
((4ug/ml) staining of Mouse SubMandibular Gland cells at E18. Nuclear counterstain with DAPI in green.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43,160 Da
NCBI Official Full Name
submandibular gland protein C
NCBI Official Synonym Full Names
submandibular gland protein C
NCBI Official Symbol
Smgc
NCBI Official Synonym Symbols
Muc19; Sfc21; DXImx49e; Muc19/Smgc; 2310010P21Rik
NCBI Protein Information
submandibular gland protein C
UniProt Protein Name
Submandibular gland protein C
UniProt Gene Name
Muc19
UniProt Synonym Gene Names
Smgc
UniProt Entry Name
SMGC_MOUSE
Uniprot Description
SMGC: 6 isoforms of the human protein are produced by alternative splicing.
Cellular Component: extracellular region
Research Articles on Smgc
Similar Products
Product Notes
The Smgc muc19 (Catalog #AAA422864) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Muc19/Smgc Antibody reacts with Tested: Mouse; Expected from sequence similarity: Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Muc19/Smgc can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunocytochemistry (ICC). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Preliminary experiments gave bands at approx 65kDa and 70kDa in Mouse fetal Lung lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 74.4kDa according to NP_945121.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). Immunocytochemistry: Positive staining of select epithelial cells in the murine Submanibular gland at E18. Recommended concentration 4ug/ml. Researchers should empirically determine the suitability of the Smgc muc19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KLEPKYENPT NGS. It is sometimes possible for the material contained within the vial of "Muc19/Smgc, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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