Western Blot (WB)
(Western Blot analysis of mtTFA on Hela cell using anti-mtTFA antibody at 1/1,000 dilution.)
Rabbit anti-Human mtTFA Polyclonal Antibody | anti-mtTFA antibody
mtTFA Polyclonal Antibody
ICC: 1:50-1:200
IF: 1:50-1:200
IHC: 1:50-1:200
IP: 1:10-1:50
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of mtTFA on Hela cell using anti-mtTFA antibody at 1/1,000 dilution.)
Western Blot (WB)
(Western Blot analysis of mtTFA on Hela cell using anti-mtTFA antibody at 1/1,000 dilution.)
Immunocytochemistry (ICC)
(ICC staining mtTFA in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining mtTFA in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a key mitochondrial transcription factor containing two high mobility group motifs. The encoded protein also functions in mitochondrial DNA replication and repair. Sequence polymorphisms in this gene are associated with Alzheimer's and Parkinson's diseases. There are pseudogenes for this gene on chromosomes 6, 7, and 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
Uniprot Description
TFAM: regulates mitochondrial transcription. Induced in humans by caloric restriction. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Activates transcription by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Involved in cisplatin resistance. Interacts with TFB1M and TFB2M.
Protein type: Transcription regulation; DNA-binding
Chromosomal Location of Human Ortholog: 10q21
Cellular Component: mitochondrion; mitochondrial matrix; cytosol; nucleus
Molecular Function: protein binding; heat shock protein binding; chromatin binding; DNA bending activity; transcription factor activity
Biological Process: mitochondrial respiratory chain complex assembly; chromatin remodeling; mitochondrion organization and biogenesis; regulation of transcription from RNA polymerase I promoter; organelle organization and biogenesis; positive regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; gene expression; DNA-dependent DNA replication; transcription from mitochondrial promoter; transcription initiation from mitochondrial promoter
Research Articles on mtTFA
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Product Notes
The mtTFA tfam (Catalog #AAA3013074) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The mtTFA Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's mtTFA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP). WB: 1:200-1:500 ICC: 1:50-1:200 IF: 1:50-1:200 IHC: 1:50-1:200 IP: 1:10-1:50. Researchers should empirically determine the suitability of the mtTFA tfam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "mtTFA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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