Rabbit MTIF2 Polyclonal Antibody | anti-MTIF2 antibody

Anti-MTIF2 Antibody

Cat. Num. AAA8307335

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry
• Purity: Purified by Immunogen Affinity Chromatography
• Synonyms: MTIF2; Polyclonal Antibody; Anti-MTIF2 Antibody; Translation initiation factor IF-2 mitochondrial; IF-2(Mt); IF-2Mt; IF2(mt); anti-MTIF2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes endogenous levels of MTIF2 protein.
• Purity/Purification: Purified by Immunogen Affinity Chromatography
• Form/Format: Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.

• Applicable Applications for anti-MTIF2 antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
• Application Notes: WB: 1:500-1:2000; IF/ICC: 1:50-1:200
• Immunogen: Recombinant fusion protein of human MTIF2. The exact sequence is proprietary.
• Preparation and Storage: Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of MTIF2 expression in A431 (A), mouse heart (B), mouse kidney (C), rat testis (D) whole cell lysates.)

Immunofluorescence (IF)/Immunocytochemistry (ICC)

(Immunofluorescent analysis of MTIF2 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.)

Related Product Information for anti-MTIF2 antibody

Rabbit polyclonal antibody to MTIF2

NCBI and Uniprot Product Information

• NCBI GI #: 53729337
• NCBI GeneID: 4528
• NCBI Accession #: NP_002444.2
• NCBI GenBank Nucleotide #: NM_002453.2
• UniProt Accession #: P46199; Q91YJ5
• Molecular Weight: 81,317 Da
• NCBI Official Full Name: translation initiation factor IF-2, mitochondrial
• NCBI Official Synonym Full Names: mitochondrial translational initiation factor 2
• NCBI Official Symbol: MTIF2
• NCBI Protein Information: translation initiation factor IF-2, mitochondrial; IF-2mt; IF2(mt); IF-2(Mt)
• UniProt Protein Name: Translation initiation factor IF-2, mitochondrial
• UniProt Gene Name: MTIF2
• UniProt Synonym Gene Names: IF-2(Mt); IF-2Mt; IF2(mt)
• UniProt Entry Name: IF2M_HUMAN

NCBI Description

During the initiation of protein biosynthesis, initiation factor-2 (IF-2) promotes the binding of the initiator tRNA to the small subunit of the ribosome in a GTP-dependent manner. Prokaryotic IF-2 is a single polypeptide, while eukaryotic cytoplasmic IF-2 (eIF-2) is a trimeric protein. Bovine liver mitochondria contain IF-2(mt), an 85-kD monomeric protein that is equivalent to prokaryotic IF-2. The predicted 727-amino acid human protein contains a 29-amino acid presequence. Human IF-2(mt) shares 32 to 38% amino acid sequence identity with yeast IF-2(mt) and several prokaryotic IF-2s, with the greatest degree of conservation in the G domains of the proteins. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

MTIF2: One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex. Belongs to the IF-2 family.

Protein type: Translation initiation; Translation; Mitochondrial

Chromosomal Location of Human Ortholog: 2p16.1

Cellular Component: nucleoplasm; mitochondrion

Molecular Function: GTPase activity; GTP binding; translation factor activity, nucleic acid binding; translation initiation factor activity; ribosomal small subunit binding

Biological Process: mitochondrial translation; organelle organization and biogenesis; ribosome disassembly; regulation of translational initiation