Mitochondrial Translational Initiation Factor 2 Recombinant Protein | MTIF2 recombinant protein

Recombinant Mitochondrial Translational Initiation Factor 2 (MTIF2)

Cat. Num. AAA2034222

• Reactivity: Rat
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >90%
• Synonyms: Mitochondrial Translational Initiation Factor 2; Recombinant Mitochondrial Translational Initiation Factor 2 (MTIF2); MTIF2 recombinant protein

• Host: E Coli
• Reactivity: Rat
• Purity/Purification: >90%
• Sequence Length: 727

• Applicable Applications for MTIF2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Fragment: Met1~Arg201
• Tag: Prokaryotic expression
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 119620510
• NCBI GeneID: 4528
• Molecular Weight: 53.1kDa
• NCBI Official Full Name: mitochondrial translational initiation factor 2, isoform CRA_b
• NCBI Official Synonym Full Names: mitochondrial translational initiation factor 2
• NCBI Official Symbol: MTIF2
• NCBI Protein Information: translation initiation factor IF-2, mitochondrial
• UniProt Protein Name: Translation initiation factor IF-2, mitochondrial
• UniProt Gene Name: MTIF2
• UniProt Synonym Gene Names: IF-2(Mt); IF-2Mt; IF2(mt)
• UniProt Entry Name: IF2M_HUMAN

NCBI Description

During the initiation of protein biosynthesis, initiation factor-2 (IF-2) promotes the binding of the initiator tRNA to the small subunit of the ribosome in a GTP-dependent manner. Prokaryotic IF-2 is a single polypeptide, while eukaryotic cytoplasmic IF-2 (eIF-2) is a trimeric protein. Bovine liver mitochondria contain IF-2(mt), an 85-kD monomeric protein that is equivalent to prokaryotic IF-2. The predicted 727-amino acid human protein contains a 29-amino acid presequence. Human IF-2(mt) shares 32 to 38% amino acid sequence identity with yeast IF-2(mt) and several prokaryotic IF-2s, with the greatest degree of conservation in the G domains of the proteins. [provided by RefSeq, Mar 2016]

Uniprot Description

MTIF2: One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex. Belongs to the IF-2 family.

Protein type: Translation; Translation initiation; Mitochondrial

Chromosomal Location of Human Ortholog: 2p16.1

Cellular Component: mitochondrion; nucleoplasm

Molecular Function: GTP binding; GTPase activity; ribosomal small subunit binding; translation factor activity, nucleic acid binding; translation initiation factor activity

Biological Process: mitochondrial translation; organelle organization and biogenesis; regulation of translational initiation; ribosome disassembly