Immunohistochemistry (IHC)
(Immunochemical staining of human MTHFD2 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Rabbit anti-Human MTHFD2 Polyclonal Antibody | anti-MTHFD2 antibody
Anti-MTHFD2 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
MTHFD2; Polyclonal Antibody; Anti-MTHFD2 Antibody; Rabbit Polyclonal; MTHFD2 Antibody; Rabbit PAb; Antigen Affinity Purified; methylenetetrahydrofolate dehydrogenase 2; methenyltetrahydrofolate cyclohydrolase; Anti-NMDMC Antibody; anti-MTHFD2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human MTHFD2
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-MTHFD2 antibody
Immunohistochemistry-Paraffin (IHC-P)
Application Notes
IHC-P: 1:50-1:200
Immunogen
E Coli-derived Human MTHFD2 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human MTHFD2 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of human MTHFD2 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human MTHFD2 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-MTHFD2 antibody
Methylenetetrahydrofolate dehydrogenase (MTHFD2), a dual-functional metabolic enzyme, is a tetramethylfolate dehydrogenase enzyme involved in folate metabolism. The folate-coupled metabolic enzyme MTHFD2 is overexpressed in many tumor types and required for cancer cell proliferation and is therefore of interest as a potential cancer therapeutic target. However, recent evidence suggests that MTHFD2 has a non-enzymatic function which may underlie the dependence of cancer cells on this protein. Methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) is specifically enhanced in cancer. MTHFD2 plays a key role in one-carbon (1C) metabolism in human mitochondria, and its high expression correlates with poor survival of patients with various types of cancer. An isozyme-selective MTHFD2 inhibitor is highly attractive for potential use in cancer treatment. MTHFD2 is a functional target gene of miR-33a-5p. miR-33a-5p inhibits the growth and migration of CRC by targeting MTHFD2.
References
Yan Y, et al. (2019) Microrna-33a-5p suppresses colorectal cancer cell growth by inhibiting mthfd2. Clin Exp Pharmacol Physiol 46 (10): 928-936.Wei Y, et al. (2019) The effect of mthfd2 on the proliferation and migration of colorectal cancer cell lines. Onco Targets Ther 12 6361-6370.Koufaris C, et al. (2018) Protein interaction and functional data indicate mthfd2 involvement in rna processing and translation. Cancer Metab 6 12.Kawai J, et al. (2019) Structure-based design and synthesis of an isozyme-selective mthfd2 inhibitor with a tricyclic coumarin scaffold. ACS Med Chem Lett 10 (6): 893-898.
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Product Notes
The MTHFD2 (Catalog #AAA8109383) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MTHFD2 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MTHFD2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-P). IHC-P: 1:50-1:200. Researchers should empirically determine the suitability of the MTHFD2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MTHFD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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