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Western Blot (WB) (Western blot analysis of extracts of various cell lines, using MTFMT antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 180s.)

Rabbit MTFMT Polyclonal Antibody | anti-MTFMT antibody

MTFMT Rabbit pAb

Gene Names
MTFMT; FMT1; COXPD15
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity purification
Synonyms
MTFMT; Polyclonal Antibody; MTFMT Rabbit pAb; FMT1; COXPD15; MC1DN27; anti-MTFMT antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Applicable Applications for anti-MTFMT antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
Recombinant protein of human MTFMT.
Positive Samples
MCF7, SH-SY5Y, Mouse brain, Rat testis
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using MTFMT antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 180s.)

Western Blot (WB) (Western blot analysis of extracts of various cell lines, using MTFMT antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 180s.)
Related Product Information for anti-MTFMT antibody
Background: The protein encoded by this nuclear gene localizes to the mitochondrion, where it catalyzes the formylation of methionyl-tRNA.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
43,832 Da
NCBI Official Full Name
methionyl-tRNA formyltransferase, mitochondrial
NCBI Official Synonym Full Names
mitochondrial methionyl-tRNA formyltransferase
NCBI Official Symbol
MTFMT
NCBI Official Synonym Symbols
FMT1; COXPD15
NCBI Protein Information
methionyl-tRNA formyltransferase, mitochondrial
UniProt Protein Name
Methionyl-tRNA formyltransferase, mitochondrial
UniProt Gene Name
MTFMT
UniProt Synonym Gene Names
FMT; FMT1; MtFMT
UniProt Entry Name
FMT_HUMAN

NCBI Description

The protein encoded by this nuclear gene localizes to the mitochondrion, where it catalyzes the formylation of methionyl-tRNA. [provided by RefSeq, Jun 2011]

Uniprot Description

MTFMT: Formylates methionyl-tRNA in mitochondria. A single tRNA(Met) gene gives rise to both an initiator and an elongator species via an unknown mechanism. Belongs to the fmt family.

Protein type: EC 2.1.2.9; Cofactor and Vitamin Metabolism - one carbon pool by folate; Mitochondrial; Methyltransferase

Chromosomal Location of Human Ortholog: 15q22.31

Cellular Component: mitochondrion

Molecular Function: methionyl-tRNA formyltransferase activity

Biological Process: mitochondrial translation; organelle organization and biogenesis

Disease: Combined Oxidative Phosphorylation Deficiency 15

Research Articles on MTFMT

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Product Notes

The MTFMT mtfmt (Catalog #AAA9143737) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MTFMT Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MTFMT can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the MTFMT mtfmt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MTFMT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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