Rabbit anti-Human Msx2 Polyclonal Antibody | anti-Msx2 antibody

Msx2, CT (Homeobox protein MSX-2, Hox-8, MSX2, HOX8) (FITC)

Cat. Num. AAA6322629

• Gene Names: MSX2; FPP; MSH; PFM; CRS2; HOX8; PFM1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Msx2; Polyclonal Antibody; CT (Homeobox protein MSX-2; Hox-8; MSX2; HOX8) (FITC); Homeobox protein MSX-2; HOX8; anti-Msx2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-Msx2 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: Msx2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human MSX2.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of Msx2 (arrow) using rabbit polyclonal Msx2 Antibody (Human C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Msx2 gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Msx2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Related Product Information for anti-Msx2 antibody

This gene encodes a member of the muscle segment homeobox gene family. The encoded protein is a transcriptional repressor whose normal activity may establish a balance between survival and apoptosis of neural crest-derived cells required for proper craniofacial morphogenesis. The encoded protein may also have a role in promoting cell growth under certain conditions and may be an important target for the RAS signaling pathways. Mutations in this gene are associated with parietal foramina 1 and craniosynostosis type 2.

Product Categories/Family for anti-Msx2 antibody

Antibodies; Transcription Factors; Zinc; Ring; Homeobox

References

Shao, J.S., Ann. N. Y. Acad. Sci. 1117, 40-50 (2007). Han, J., Mech. Dev. 124 (9-10), 729-745 (2007). Ghassibe, M., Eur. J. Pediatr. 165 (10), 734-735 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 27886557
• NCBI GeneID: 4488
• NCBI Accession #: NP_002440.2
• NCBI GenBank Nucleotide #: NM_002449.4
• UniProt Accession #: P35548; Q53XM4; Q9UD60; D3DQN1
• Molecular Weight: 28,897 Da
• NCBI Official Full Name: homeobox protein MSX-2
• NCBI Official Synonym Full Names: msh homeobox 2
• NCBI Official Symbol: MSX2
• NCBI Official Synonym Symbols: FPP; MSH; PFM; CRS2; HOX8; PFM1
• NCBI Protein Information: homeobox protein MSX-2; msh homeo box 2; homeobox protein Hox-8; msh homeobox homolog 2
• UniProt Protein Name: Homeobox protein MSX-2
• Protein Family: Msx2-interacting protein
• UniProt Gene Name: MSX2
• UniProt Synonym Gene Names: HOX8
• UniProt Entry Name: MSX2_HUMAN

NCBI Description

This gene encodes a member of the muscle segment homeobox gene family. The encoded protein is a transcriptional repressor whose normal activity may establish a balance between survival and apoptosis of neural crest-derived cells required for proper craniofacial morphogenesis. The encoded protein may also have a role in promoting cell growth under certain conditions and may be an important target for the RAS signaling pathways. Mutations in this gene are associated with parietal foramina 1 and craniosynostosis type 2. [provided by RefSeq, Jul 2008]

Uniprot Description

MSX2: Acts as a transcriptional regulator in bone development. Represses the ALPL promoter activity and antogonizes the stimulatory effect of DLX5 on ALPL expression during osteoblast differentiation. Probable morphogenetic role. May play a role in limb-pattern formation. In osteoblasts, suppresses transcription driven by the osteocalcin FGF response element (OCFRE). Binds to the homeodomain-response element of the ALPL promoter. Defects in MSX2 are the cause of parietal foramina 1 (PFM1); also known as foramina parietalia permagna (FPP). PFM1 is an autosomal dominant disease characterized by oval defects of the parietal bones caused by deficient ossification around the parietal notch, which is normally obliterated during the fifth fetal month. Defects in MSX2 are the cause of parietal foramina with cleidocranial dysplasia (PFMCCD); also known as cleidocranial dysplasia with parietal foramina. PFMCCD combines skull defects in the form of enlarged parietal foramina and deficient ossification of the clavicles. Defects in MSX2 are the cause of craniosynostosis type 2 (CRS2); also known as craniosynostosis Boston-type (CSB). CRS2 is an autosomal dominant disorder characterized by the premature fusion of calvarial sutures. The craniosynostosis phenotype is either fronto-orbital recession, or frontal bossing, or turribrachycephaly, or cloverleaf skull. Associated features include severe headache, high incidence of visual problems (myopia or hyperopia), and short first metatarsals. Intelligence is normal. Belongs to the Msh homeobox family.

Protein type: DNA-binding; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 5q35.2

Cellular Component: transcription factor complex; nucleus

Molecular Function: protein binding; sequence-specific DNA binding; transcription cofactor activity; transcription factor binding

Biological Process: embryonic forelimb morphogenesis; transcription from RNA polymerase II promoter; negative regulation of keratinocyte differentiation; negative regulation of transcription from RNA polymerase II promoter; chondrocyte development; negative regulation of fat cell differentiation; positive regulation of catagen; embryonic hindlimb morphogenesis; osteoblast development; osteoblast differentiation; anterior/posterior pattern formation; negative regulation of cell proliferation; positive regulation of osteoblast differentiation; inhibition of CREB transcription factor; negative regulation of transcription, DNA-dependent; positive regulation of BMP signaling pathway; wound healing, spreading of epidermal cells; negative regulation of apoptosis

Disease: Parietal Foramina; Parietal Foramina With Cleidocranial Dysplasia; Craniosynostosis 2