Rabbit Mst2 Polyclonal Antibody | anti-Mst2 antibody

Mst2 Polyclonal Antibody

Cat. Num. AAA3013071

• Gene Names: STK3; KRS1; MST2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: ProA affinity purified
• Synonyms: Mst2; Polyclonal Antibody; Mst2 Polyclonal Antibody; MST-2; STK3; KRS1; MST2; DKFZp686A2068; FLJ90748; KRS2; Mammalian STE20 like protein kinase 1; Mammalian STE20 like protein kinase 2; MST 1; MST 2; MST1; Serine/threonine kinase 3 (STE20 homolog yeast); Serine/threonine kinase 3 (Ste20 yeast homolog); Serine/threonine kinase 3; Serine/threonine kinase 4; Serine/threonine protein kinase 3; Serine/threonine protein kinase 4; Serine/threonine protein kinase Krs 1; Serine/threonine protein kinase Krs 2; STE20 like kinase MST1; STE20 like kinase MST2; STK 3; STK 4; STK4; YSK3; anti-Mst2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Mst2 polyclonal antibody detects endogenous levels of Mst2 protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 519

• Applicable Applications for anti-Mst2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: WB: 1:1,000-1:5,000; IHC: 1:50-1:200
• Immunogen: recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of Mst2 on different lysates using anti-Mst2 antibody at 1/1,000 dilution. Positive control: Lane 1: CRC Lane 2: HCT116)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-Mst2 antibody. Counter stained with hematoxylin.)

Related Product Information for anti-Mst2 antibody

Sterile-20 (Ste20) is a serine/threonine kinase in Saccharomyces cerevisiae that is involved in relaying signals from G protein-coupled receptors to cyto-solic MAP kinase cascades. Mammalian protein kinases that display sequence similarity to Ste20 are divided into two groups, the PAK subfamily and the GCK subfamily. The PAK subfamily members contain a C-terminal catalytic domain and an N-terminal regulatory domain with a p21Rac/Cdc42-binding site, and these kinases can activate both p38 MAPK and JNK. The GCK subfamily members contain a C-terminal regulatory domain and an N-terminal catalytic domain, and they have diverse roles in many pathways, including the activation of ERK, JNK, p38 MAPK, and caspase-3. The mammalian Ste20-like kinases (MST kinases), also known as Krs proteins, are members of the GCK subfamily. Ksr-1 (MST-2) and Ksr-2 (MST-1) are both direct substrates of caspase-3 that accelerate caspase-3 activation. MST-3 is ubiquitously expressed in mammalian tissue and can phosphorylate exogenous substrates as well as itself. MST-4 is highly expressed in placenta, thymus, and peripheral blood leukocytes, and it specifically activates ERK.

NCBI and Uniprot Product Information

• NCBI GI #: 372622373
• NCBI GeneID: 6788
• NCBI Accession #: NP_001243241.1
• NCBI GenBank Nucleotide #: NP_001243241.1
• UniProt Accession #: Q13188; Q9JI11; O54748
• Molecular Weight: 36/56kDa
• NCBI Official Full Name: serine/threonine-protein kinase 3 isoform 2
• NCBI Official Synonym Full Names: serine/threonine kinase 3
• NCBI Official Symbol: STK3
• NCBI Official Synonym Symbols: KRS1; MST2
• NCBI Protein Information: serine/threonine-protein kinase 3
• UniProt Protein Name: Serine/threonine-protein kinase 3
• Protein Family: Mst2 complex
• UniProt Gene Name: STK3
• UniProt Synonym Gene Names: KRS1; MST2; MST-2; MST2/N; MST2/C

NCBI Description

This gene encodes a serine/threonine protein kinase activated by proapoptotic molecules indicating the encoded protein functions as a growth suppressor. Cleavage of the protein product by caspase removes the inhibitory C-terminal portion. The N-terminal portion is transported to the nucleus where it homodimerizes to form the active kinase which promotes the condensation of chromatin during apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

MST2: a protein kinase of the STE20 family. Activated by apoptotic signals as well as other stress conditions. Full activation requires both phosphorylation and caspase-mediated cleavage. Following cleavage by caspase-3, undergoes irreversible autophosphorylation. Full-length is mainly unphosphorylated in the rat thymus and cultured cells, whereas caspase-3-cleaved MST2 in apoptotic cells is highly phosphorylated.

Protein type: Autophagy; EC 2.7.11.1; Kinase, protein; MST subfamily; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family

Chromosomal Location of Human Ortholog: 8q22.2

Cellular Component: cytoplasm; cytosol; protein complex

Molecular Function: ATP binding; magnesium ion binding; protein binding; protein dimerization activity; protein kinase activity; protein serine/threonine kinase activator activity; protein serine/threonine kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: apoptosis; positive regulation of apoptosis; positive regulation of protein binding; positive regulation of transcription factor activity; protein amino acid phosphorylation; protein stabilization; signal transduction