Rabbit MST1R Polyclonal Antibody | anti-MST1R antibody

MST1R Antibody (Center)

Cat. Num. AAA9231099

• Gene Names: MST1R; RON; PTK8; CD136; NPCA3; CDw136
• Reactivity: Human; Predicted: Human
• Applications: Western Blot, ELISA
• Synonyms: MST1R; Polyclonal Antibody; MST1R Antibody (Center); Macrophage-stimulating protein receptor; MSP receptor; 2.7.10.1; CDw136; Protein-tyrosine kinase 8; p185-Ron; CD136; Macrophage-stimulating protein receptor alpha chain; Macrophage-stimulating protein receptor beta chain; PTK8; RON; anti-MST1R antibody

• Host: Rabbit
• Reactivity: Human; Predicted: Human
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This MST1R antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 600-633 amino acids from the Central region of human MST1R.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 1351

• Applicable Applications for anti-MST1R antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000-1:2000
• Antigen Type: Synthesized Peptide
• Antigen Source: Human
• Cellular Location: Membrane; Single-pass type I membrane protein
• Tissue Location: Expressed in colon, skin, lung and bone marrow.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-MST1R Antibody (Center) at 1:1000-1:2000 dilutionLane 1: HACAT whole cell lysateLane 2: HCT116 whole cell lysateLane 3: SK-BR-3 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 152 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-MST1R antibody

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to MST1 ligand. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces autophosphorylation of RON on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1 or the adapter GAB1. Recruitment of these downstream effectors by RON leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. RON signaling activates the wound healing response by promoting epithelial cell migration, proliferation as well as survival at the wound site. Plays also a role in the innate immune response by regulating the migration and phagocytic activity of macrophages. Alternatively, RON can also promote signals such as cell migration and proliferation in response to growth factors other than MST1 ligand.

Product Categories/Family for anti-MST1R antibody

Primary Antibodies; Cell Biology; Signal Transduction

References

Ronsin C.,et al.Oncogene 8:1195-1202 (1993). Collesi C.,et al.Mol. Cell. Biol. 16:5518-5526 (1996). Jin P.,et al.Arthritis Res. Ther. 10:R73-R73 (2008). Muzny D.M.,et al.Nature 440:1194-1198 (2006). Ponzetto C.,et al.Mol. Cell. Biol. 13:4600-4608 (1993).

NCBI and Uniprot Product Information

• NCBI GI #: 349732251
• NCBI GeneID: 4486
• NCBI Accession #: NP_001231866.1
• NCBI GenBank Nucleotide #: NM_001244937.2
• UniProt Accession #: Q04912; B5A944; B5A945; B5A946; B5A947
• Molecular Weight: 152271 Da
• NCBI Official Full Name: macrophage-stimulating protein receptor isoform 2
• NCBI Official Synonym Full Names: macrophage stimulating 1 receptor
• NCBI Official Symbol: MST1R
• NCBI Official Synonym Symbols: RON; PTK8; CD136; NPCA3; CDw136
• NCBI Protein Information: macrophage-stimulating protein receptor
• UniProt Protein Name: Macrophage-stimulating protein receptor
• Protein Family: Macrophage-stimulating protein receptor
• UniProt Gene Name: MST1R
• UniProt Synonym Gene Names: PTK8; RON; MSP receptor

NCBI Description

This gene encodes a cell surface receptor for macrophage-stimulating protein (MSP) with tyrosine kinase activity. The mature form of this protein is a heterodimer of disulfide-linked alpha and beta subunits, generated by proteolytic cleavage of a single-chain precursor. The beta subunit undergoes tyrosine phosphorylation upon stimulation by MSP. This protein is expressed on the ciliated epithelia of the mucociliary transport apparatus of the lung, and together with MSP, thought to be involved in host defense. Alternative splicing generates multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Jan 2016]

Uniprot Description

Ron: a receptor tyrosine kinase of the Met family. Receptor for macrophage stimulating protein. Can be desensitized by c-Cbl and its binding partner Grb2. Overexpression of wild-type RON causes the formation of lung tumors. Two alternatively spliced isoforms have been described.

Protein type: EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Met family; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: cell surface; integral to plasma membrane; plasma membrane

Molecular Function: enzyme binding; macrophage colony stimulating factor receptor activity; protein binding

Biological Process: cell motility; defense response; hepatocyte growth factor receptor signaling pathway; positive regulation of cell proliferation; positive regulation of MAP kinase activity; positive regulation of protein kinase B signaling cascade; response to virus; signal transduction; single fertilization

Disease: Nasopharyngeal Carcinoma, Susceptibility To, 3