MSK1 Polyclonal Antibody | anti-MSK1 antibody

Anti-MSK1 Antibody

Cat. Num. AAA177737

• Gene Names: RPS6KA5; MSK1; RLPK; MSPK1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: MSK1; Polyclonal Antibody; Anti-MSK1 Antibody; Ribosomal protein S6 kinase alpha-5; 90 kDa ribosomal protein S6 kinase 5; KS6A5_HUMAN; MGC1911; Mitogen and stress activated protein kinase 1; MSPK1; Nuclear Mitogen And Stress Activated Protein Kinase 1; Nuclear mitogen- and stress-activated protein kinase 1; Ribosomal protein S6 kinase 90kD polypeptide 5; Ribosomal protein S6 kinase 90kDa; Ribosomal protein S6 kinase 90kDa polypeptide 5; Ribosomal Protein S6 Kinase Alpha 5; RLPK; RPS6KA5; RSK Like Protein Kinase; RSK-like protein kinase; RSKL; S6K alpha 5; S6K-alpha-5; ribosomal protein S6 kinase; 90kDa; polypeptide 5; anti-MSK1 antibody

• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 802

• Applicable Applications for anti-MSK1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC)
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml; Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml; Immunocytochemistry Concentration: 0.5-1ug/ml
• Immunogen: E Coli-derived human MSK1 recombinant protein (Position: V540-Q665). Human MSK1 shares 98% amino acid (aa) sequence identity with mouse MSK1.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- MSK1 Picoband antibody, MBS177737, Western blottingAll lanes: Anti MSK1 (MBS177737) at 0.5ug/mlWB: Recombinant Human MSK1 Protein 0.5ngPredicted bind size: 50KDObserved bind size: 50KD )

Western Blot (WB)

(Anti- MSK1 Picoband antibody, MBS177737, Western blottingAll lanes: Anti MSK1 (MBS177737) at 0.5ug/mlLane 1: Human Placenta Tissue Lysate at 50ugLane 2: Rat Brain Tissue Lysate at 50ugLane 3: JURKAT Whole Cell Lysate at 40ugLane 4: HELA Whole Cell Lysate at 40ugLane 5: PANC Whole Cell Lysate at 40ugLane 6: A549 Whole Cell Lysate at 40ugLane 7: HEPG2 Whole Cell Lysate at 40ugPredicted bind size: 90KDObserved bind size: 90KD )

Immunohistochemistry (IHC)

(Anti- MSK1 Picoband antibody, MBS177737, IHC(P)IHC(P): Mouse Cardiac Muscle Tissue )

Immunohistochemistry (IHC)

(Anti- MSK1 Picoband antibody, MBS177737, IHC(P)IHC(P): Rat Brain Tissue )

Immunohistochemistry (IHC)

(Anti- MSK1 Picoband antibody, MBS177737, IHC(P)IHC(P): Human Placenta Tissue )

Related Product Information for anti-MSK1 antibody

Description: Rabbit IgG polyclonal antibody for Ribosomal protein S6 kinase alpha-5(RPS6KA5) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.

Background: Ribosomal protein S6 kinase alpha-5, also known as MSK1, is an enzyme that in humans is encoded by the RPS6KA5 gene. It belongs to the AGC family of kinases. MSK1 gene is mapped to chromosome 14q31-q32. Northern blot analysis indicated that MSK1 was expressed as a 4-kb mRNA in all tissues tested, with the highest levels of expression in brain, muscle, and placenta. MSK1 was activated in vitro and in vivo by either ERK or SAPK2 proteins. It has been found that MSK1, rather than MAPKAP-K1 or MAPKAP-K2/K3, mediates activation of the cAMP response element-binding protein and activating transcription factor-1 by either growth factors or stress signals.

References

1. Deak, M., Clifton, A. D., Lucocq, J. M., Alessi, D. R. Mitogen- and stress-activated protein kinase-1 (MSK1) is directly activated by MAPK and SAPK2/p38, and may mediate activation of CREB. EMBO J. 17: 4426-4441, 1998. 2. Jiang, C., Yu, L., Tu, Q., Zhao, Y., Zhang, H., Zhao, S. Assignment of a member of the ribosomal protein S6 kinase family, RPS6KA5, to human chromosome 14q31-q32.1 by radiation hybrid mapping. Cytogenet. Cell Genet. 87: 261-261, 1999.

NCBI and Uniprot Product Information

• NCBI GI #: 32528295
• NCBI GeneID: 9252
• NCBI Accession #: NP_004746.2
• NCBI GenBank Nucleotide #: NM_004755.3
• UniProt Accession #: O75582; O95316; Q96AF7; B7Z2Y5
• Molecular Weight: 81,780 Da
• NCBI Official Full Name: ribosomal protein S6 kinase alpha-5 isoform a
• NCBI Official Synonym Full Names: ribosomal protein S6 kinase A5
• NCBI Official Symbol: RPS6KA5
• NCBI Official Synonym Symbols: MSK1; RLPK; MSPK1
• NCBI Protein Information: ribosomal protein S6 kinase alpha-5
• UniProt Protein Name: Ribosomal protein S6 kinase alpha-5
• UniProt Gene Name: RPS6KA5
• UniProt Synonym Gene Names: MSK1; S6K-alpha-5; RSKL
• UniProt Entry Name: KS6A5_HUMAN

Uniprot Description

MSK1: an AGC kinase of the RSK family. Has two kinase domains connected by a regulatory linker region. Enzyme activity requires the presence of both kinase domains. Is phosphorylated and activated by Erk as well as p38 MAPK in response to growth factors and cellular stress.

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Kinase, protein; Protein kinase, AGC; AGC group; RSK family; MSK subfamily

Chromosomal Location of Human Ortholog: 14q31-q32.1

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function: ATP binding; histone serine kinase activity (H3-S10 specific); magnesium ion binding; protein binding; protein kinase activity; protein serine/threonine kinase activity

Biological Process: activation of CREB transcription factor; activation of NF-kappaB transcription factor; axon guidance; epidermal growth factor receptor signaling pathway; histone phosphorylation; inflammatory response; negative regulation of cytokine production; negative regulation of transcription, DNA-dependent; positive regulation of histone acetylation; positive regulation of histone phosphorylation; positive regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; regulation of transcription, DNA-dependent; stimulatory C-type lectin receptor signaling pathway