Western Blot (WB)
(Western Blot analysis of mSin3A on 293T cell using anti-mSin3A antibody at 1/1,000 dilution.)
Rabbit mSin3A Polyclonal Antibody | anti-mSin3A antibody
mSin3A Polyclonal Antibody
Gene Names
SIN3A; WITKOS
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
mSin3A; Polyclonal Antibody; mSin3A Polyclonal Antibody; SIN3A; AW553200; DKFZP434K2235; FLJ90319; Histone deacetylase complex subunit Sin 3a; Histone deacetylase complex subunit Sin3a; KIAA0700; Kiaa4126; mKIAA4126; Paired amphipathic helix protein Sin 3a; Paired amphipathic helix protein Sin3a; Sin 3a; SIN3 homolog A; SIN3 homolog A transcription regulator (yeast); SIN3 homolog A transcription regulator; SIN3 transcription regulator homolog A; Sin3a; SIN3A protein; Transcriptional co repressor Sin 3A; Transcriptional co repressor Sin3A; Transcriptional corepressor Sin3a; Transcriptional regulator SIN3A; anti-mSin3A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
mSin3A polyclonal antibody detects endogenous levels of mSin3A protein.
Purity/Purification
ProA affinity purified
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
1273
Applicable Applications for anti-mSin3A antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:2,000
ICC: 1:50-1:200
IHC: 1:50
FC/FACS: 1:50-1:100
ICC: 1:50-1:200
IHC: 1:50
FC/FACS: 1:50-1:100
Immunogen
recombinant protein
Preparation and Storage
Store at 4 degree C after thawing.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of mSin3A on 293T cell using anti-mSin3A antibody at 1/1,000 dilution.)
Western Blot (WB)
(Western Blot analysis of mSin3A on 293T cell using anti-mSin3A antibody at 1/1,000 dilution.)
Immunocytochemistry (ICC)
(ICC staining mSin3A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining mSin3A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Related Product Information for anti-mSin3A antibody
It is now well established that Myc regulation of cell proliferation and differentiation involves a family of related transcription factors. One such factor, Max, is an obligate heterodimeric partner for Myc and can also form heterodimers with at least four related proteins designated Mad 1, Mxi1 (alternatively designated Mad 2), Mad 3 and Mad 4. Like Mad 1 and Mxi1, association of Mad 3 and Mad 4 with Max results in transcriptional repression. Both Myc and the Mad proteins have short half-lives and their synthesis is tightly regulated, while Max expression is constitutive and relatively stable. Two related mammalian cDNAs have been identified and shown to encode Mad-binding proteins. Both possess sequence homology with the yeast transcription repressor Sin3 including four conserved paired amphipathic helix (PAH) domains. mSin3A and mSin3B specifically interact with the Mad proteins via their second paired amphipathic helix domain (PAH2). It has been suggested that Mad-Max heterodimers repress transcription by tethering mSin3 to DNA as corepressors.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
145kDa
NCBI Official Full Name
paired amphipathic helix protein Sin3a
NCBI Official Synonym Full Names
SIN3 transcription regulator family member A
NCBI Official Symbol
SIN3A
NCBI Official Synonym Symbols
WITKOS
NCBI Protein Information
paired amphipathic helix protein Sin3a
UniProt Protein Name
Paired amphipathic helix protein Sin3a
UniProt Gene Name
SIN3A
UniProt Entry Name
SIN3A_HUMAN
NCBI Description
The protein encoded by this gene is a transcriptional regulatory protein. It contains paired amphipathic helix (PAH) domains, which are important for protein-protein interactions and may mediate repression by the Mad-Max complex. [provided by RefSeq, Jul 2008]
Research Articles on mSin3A
Similar Products
Product Notes
The mSin3A sin3a (Catalog #AAA3013070) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The mSin3A Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's mSin3A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500-1:2,000 ICC: 1:50-1:200 IHC: 1:50 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the mSin3A sin3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "mSin3A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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