Goat MSI2/musashi-2 Polyclonal Antibody | anti-MSI2 antibody

Goat anti-MSI2/musashi-2 (aa33-43) Antibody

Cat. Num. AAA423194

• Gene Names: MSI2; MSI2H
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Cow
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: MSI2/musashi-2; Polyclonal Antibody; Goat anti-MSI2/musashi-2 (aa33-43) Antibody; Goat Anti-MSI2 / musashi-2 Antibody; MSI2; musashi RNA-binding protein 2; MSI2H; RNA-binding protein Musashi homolog 2; musashi homolog 2; musashi-2; MSI2 antibody; musashi RNA-binding protein 2 antibody; MSI2H antibody; RNA-binding protein Musashi homolog 2 antibody; musashi homolog 2 antibody; musashi-2 antibody; Musashi-2; MSI2 / musashi-2 (aa33-43); anti-MSI2 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: SPDSLRDYFSK
• Sequence Length: 328

• Applicable Applications for anti-MSI2 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 128000.; Western Blot: Approx 37kDa band observed in lysates of cell line Kelly (calculated MW of 35.2kDa according to NP_620412.1). Recommended concentration: 0.1-0.3ug/ml.
• Immunogen: Peptide with sequence C-SPDSLRDYFSK, from the internal region of the protein sequence according to NP_620412.1; NP_733839.1.
• Epitope: Internal region
• Note: This antibody is expected to recognize both reported isoforms (NP_620412.1; NP_733839.1).
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.1ug/ml) staining of Kelly lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB)

((0.3ug/ml) staining of fetal Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 20373175
• NCBI GeneID: 124540
• NCBI Accession #: NP_620412.1; NP_733839.1
• NCBI GenBank Nucleotide #: NM_138962.3
• Molecular Weight: 17,238 Da
• NCBI Official Full Name: RNA-binding protein Musashi homolog 2 isoform a
• NCBI Official Synonym Full Names: musashi RNA binding protein 2
• NCBI Official Symbol: MSI2
• NCBI Official Synonym Symbols: MSI2H
• NCBI Protein Information: RNA-binding protein Musashi homolog 2
• UniProt Protein Name: RNA-binding protein Musashi homolog 2
• Protein Family: RNA-binding protein
• UniProt Gene Name: MSI2
• UniProt Synonym Gene Names: Musashi-2
• UniProt Entry Name: MSI2H_HUMAN

NCBI Description

This gene encodes an RNA-binding protein that is a member of the Musashi protein family. The encoded protein is transcriptional regulator that targets genes involved in development and cell cycle regulation. Mutations in this gene are associated with poor prognosis in certain types of cancers. This gene has also been shown to be rearranged in certain cancer cells. [provided by RefSeq, Apr 2016]

Uniprot Description

Musashi-2: RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system. Chromosomal aberrations involving MSI2 may contribute to disease progression in chronic myeloid leukemia. Translocation t(7;17)(p15;q23) with HOXA9; translocation t(7;17)(q32-34;q23). Belongs to the Musashi family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 17q22

Cellular Component: cytoplasm; polysome

Molecular Function: nucleotide binding; poly(U) binding; protein binding

Biological Process: stem cell development