Rabbit anti-Human MRPS24 Polyclonal Antibody | anti-MRPS24 antibody

MRPS24, ID (MRPS24, 28S ribosomal protein S24, mitochondrial, bMRP-47) (PE)

Cat. Num. AAA6322339

• Gene Names: MRPS24; S24mt; bMRP47; HSPC335; MRP-S24; bMRP-47
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MRPS24; Polyclonal Antibody; ID (MRPS24; 28S ribosomal protein S24; mitochondrial; bMRP-47) (PE); bMRP-47; anti-MRPS24 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-MRPS24 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: MRPS24 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-58 amino acids from the Central region of human MRPS24.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(MRPS24 Antibody (Center) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the MRPS24 antibody detected the MRPS24 protein (arrow).)

Immunohistochemistry (IHC)

(MRPS24 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MRPS24 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(MRPS24 Antibody (Center) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-MRPS24 antibody

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPS24 encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 11. [provided by RefSeq].

Product Categories/Family for anti-MRPS24 antibody

Antibodies; Mitochondrial Proteins

References

Lamesch, P., et al. Genomics 89(3):307-315(2007). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007): Zhang, Z., et al. Genomics 81(5):468-480(2003). Kenmochi, N., et al. Genomics 77 (1-2), 65-70 (2001): Suzuki, T., et al. J. Biol. Chem. 276(35):33181-33195(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 15721937
• NCBI GeneID: 64951
• NCBI Accession #: NP_114403.1
• NCBI GenBank Nucleotide #: NM_032014.2
• UniProt Accession #: Q96EL2; P82668; Q96Q23; Q9P047; A4D1U9
• Molecular Weight: 19,015 Da
• NCBI Official Full Name: 28S ribosomal protein S24, mitochondrial
• NCBI Official Synonym Full Names: mitochondrial ribosomal protein S24
• NCBI Official Symbol: MRPS24
• NCBI Official Synonym Symbols: S24mt; bMRP47; HSPC335; MRP-S24; bMRP-47
• NCBI Protein Information: 28S ribosomal protein S24, mitochondrial; mitochondrial 28S ribosomal protein S24
• UniProt Protein Name: 28S ribosomal protein S24, mitochondrial
• Protein Family: 28S ribosomal protein
• UniProt Gene Name: MRPS24
• UniProt Synonym Gene Names: MRP-S24; S24mt; bMRP47
• UniProt Entry Name: RT24_HUMAN

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 11. Read-through transcription exists between this gene and the upstream upregulator of cell proliferation (URGCP) gene. [provided by RefSeq, Mar 2011]

Uniprot Description

MRPS24: Component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 7p14

Cellular Component: mitochondrial inner membrane; mitochondrial small ribosomal subunit; mitochondrial large ribosomal subunit

Molecular Function: structural constituent of ribosome

Biological Process: translation; mitochondrial translation; organelle organization and biogenesis