Rabbit MRPS12 Polyclonal Antibody | anti-MRPS12 antibody

MRPS12 Polyclonal Antibody

Cat. Num. AAA9131062

• Gene Names: MRPS12; RPS12; RPMS12; RPSM12; MPR-S12; MT-RPS12
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purification
• Synonyms: MRPS12; Polyclonal Antibody; MRPS12 Polyclonal Antibody; MPR-S12; MT-RPS12; RPMS12; RPS12; RPSM12; anti-MRPS12 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Sequence Length: 138

• Applicable Applications for anti-MRPS12 antibody: Western Blot (WB)
• Application Notes: WB: 1:1000 - 1:2000
• Immunogen: Recombinant protein of human MRPS12
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using MRPS12 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)

Related Product Information for anti-MRPS12 antibody

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S12P family. The encoded protein is a key component of the ribosomal small subunit and controls the decoding fidelity and susceptibility to aminoglycoside antibiotics. The gene for mitochondrial seryl-tRNA synthetase is located upstream and adjacent to this gene, and both genes are possible candidates for the autosomal dominant deafness gene (DFNA4). Splice variants that differ in the 5' UTR have been found for this gene; all three variants encode the same protein.

Product Categories/Family for anti-MRPS12 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 3024582
• NCBI GeneID: 6183
• UniProt Accession #: O15235; Q53X98
• Molecular Weight: 15kDa
• NCBI Official Full Name: 28S ribosomal protein S12, mitochondrial
• NCBI Official Synonym Full Names: mitochondrial ribosomal protein S12
• NCBI Official Symbol: MRPS12
• NCBI Official Synonym Symbols: RPS12; RPMS12; RPSM12; MPR-S12; MT-RPS12
• NCBI Protein Information: 28S ribosomal protein S12, mitochondrial
• UniProt Protein Name: 28S ribosomal protein S12, mitochondrial
• Protein Family: 28S ribosomal protein
• UniProt Gene Name: MRPS12
• UniProt Synonym Gene Names: RPMS12; RPSM12; MRP-S12; S12mt

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S12P family. The encoded protein is a key component of the ribosomal small subunit and controls the decoding fidelity and susceptibility to aminoglycoside antibiotics. The gene for mitochondrial seryl-tRNA synthetase is located upstream and adjacent to this gene, and both genes are possible candidates for the autosomal dominant deafness gene (DFNA4). Splice variants that differ in the 5' UTR have been found for this gene; all three variants encode the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

MRPS12: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S12P family. The encoded protein is a key component of the ribosomal small subunit and controls the decoding fidelity and susceptibility to aminoglycoside antibiotics. The gene for mitochondrial seryl-tRNA synthetase is located upstream and adjacent to this gene, and both genes are possible candidates for the autosomal dominant deafness gene (DFNA4). Splice variants that differ in the 5' UTR have been found for this gene; all three variants encode the same protein. [provided by RefSeq, Jul 2008]

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: mitochondrial inner membrane; mitochondrial ribosome; mitochondrial small ribosomal subunit

Molecular Function: protein binding; RNA binding; structural constituent of ribosome

Biological Process: mitochondrial translation; translation