Rabbit anti-Human MRPS12 Polyclonal Antibody | anti-MRPS12 antibody

MRPS12, ID (K43) (MRPS12, RPMS12, RPSM12, 28S ribosomal protein S12, mitochondrial, MT-RPS12) (APC)

Cat. Num. AAA6322286

• Gene Names: MRPS12; RPS12; RPMS12; RPSM12; MPR-S12; MT-RPS12
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MRPS12; Polyclonal Antibody; ID (K43) (MRPS12; RPMS12; RPSM12; 28S ribosomal protein S12; mitochondrial; MT-RPS12) (APC); MT-RPS12; anti-MRPS12 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 138

• Applicable Applications for anti-MRPS12 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: MRPS12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 35-67 amino acids from the Central region of human MRPS12.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(MRPS12 Antibody (Center K43) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the MRPS12 antibody detected the MRPS12 protein (arrow).)

Immunohistochemistry (IHC)

(MRPS12 Antibody (Center K43) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MRPS12 Antibody (Center K43) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-MRPS12 antibody

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S12P family. The encoded protein is a key component of the ribosomal small subunit and controls the decoding fidelity and susceptibility to aminoglycoside antibiotics. The gene for mitochondrial seryl-tRNA synthetase is located upstream and adjacent to this gene, and both genes are possible candidates for the autosomal dominant deafness gene (DFNA4). Splice variants that differ in the 5' UTR have been found for this gene; all three variants encode the same protein.

Product Categories/Family for anti-MRPS12 antibody

Antibodies; Mitochondrial Proteins

References

Zanotto, E., et al. Biochim. Biophys. Acta 1789(5):432-442(2009). Russo, A., et al. Biochim. Biophys. Acta 1779(12):820-829(2008). Stelzl, U., et al. Cell 122(6):957-968(2005). Cui, Y.P., et al. World J. Gastroenterol. 9(9):1892-1896(2003). Zhang, Z., et al. Genomics 81(5):468-480(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 16950589
• NCBI GeneID: 6183
• NCBI Accession #: NP_203526.1; NP_066930.1
• NCBI GenBank Nucleotide #: NP_203526.1
• UniProt Accession #: O15235
• NCBI Official Full Name: 28S ribosomal protein S12, mitochondrial
• NCBI Official Synonym Full Names: mitochondrial ribosomal protein S12
• NCBI Official Symbol: MRPS12
• NCBI Official Synonym Symbols: RPS12; RPMS12; RPSM12; MPR-S12; MT-RPS12
• NCBI Protein Information: 28S ribosomal protein S12, mitochondrial
• UniProt Protein Name: 28S ribosomal protein S12, mitochondrial
• Protein Family: 28S ribosomal protein
• UniProt Gene Name: MRPS12
• UniProt Synonym Gene Names: RPMS12; RPSM12; MRP-S12; S12mt
• UniProt Entry Name: RT12_HUMAN

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S12P family. The encoded protein is a key component of the ribosomal small subunit and controls the decoding fidelity and susceptibility to aminoglycoside antibiotics. The gene for mitochondrial seryl-tRNA synthetase is located upstream and adjacent to this gene, and both genes are possible candidates for the autosomal dominant deafness gene (DFNA4). Splice variants that differ in the 5' UTR have been found for this gene; all three variants encode the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

MRPS12: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S12P family. The encoded protein is a key component of the ribosomal small subunit and controls the decoding fidelity and susceptibility to aminoglycoside antibiotics. The gene for mitochondrial seryl-tRNA synthetase is located upstream and adjacent to this gene, and both genes are possible candidates for the autosomal dominant deafness gene (DFNA4). Splice variants that differ in the 5' UTR have been found for this gene; all three variants encode the same protein. [provided by RefSeq, Jul 2008]

Chromosomal Location of Human Ortholog: 19q13.1-q13.2

Cellular Component: small ribosomal subunit; mitochondrial inner membrane; mitochondrial ribosome

Molecular Function: protein binding; structural constituent of ribosome

Biological Process: mitochondrial translation; organelle organization and biogenesis