Rabbit MRPS12 Polyclonal Antibody | anti-MRPS12 antibody

MRPS12 Antibody

Cat. Num. AAA9212549

• Gene Names: MRPS12; RPS12; RPMS12; RPSM12; MPR-S12; MT-RPS12
• Reactivity: Human, mouse, rat
• Applications: Western Blot, ELISA
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: MRPS12; Polyclonal Antibody; MRPS12 Antibody; 28S ribosomal protein S12; mitochondrial; MRP-S12; S12mt; MT-RPS12; RPMS12; RPSM12; anti-MRPS12 antibody

• Host: Rabbit
• Reactivity: Human, mouse, rat
• Clonality: Polyclonal
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: 0.01M PBS, pH 7.2, 0.1% Sodium azide, Glycerol 50%
• Concentration: 1mg/ml (varies by lot)
• Sequence Positions: 1-60
• Sequence Length: 138

• Applicable Applications for anti-MRPS12 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~ 1:4000
• Antigen Type: synthetic peptide
• Antigen Source: KLH conjugated synthetic peptide derived from human MRPS12
• Preparation and Storage: Store at -20 degree C.Stable for 12 months from date of receipt

Western Blot (WB)

(Anti-MRPS12 Antibodyat 1:4000 dilution + Jurkat whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 15 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

References

Shah Z.H.,et al.Gene 204:55-62(1997).
Johnson D.F.,et al.Genomics 52:363-368(1998).
Ebert L.,et al.Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Burkard T.R.,et al.BMC Syst. Biol. 5:17-17(2011).

NCBI and Uniprot Product Information

• NCBI GI #: 11056056
• NCBI GeneID: 6183
• NCBI Accession #: NP_066930.1
• NCBI GenBank Nucleotide #: NM_021107.1
• UniProt Accession #: O15235; Q53X98
• Molecular Weight: 15173
• NCBI Official Full Name: 28S ribosomal protein S12, mitochondrial
• NCBI Official Synonym Full Names: mitochondrial ribosomal protein S12
• NCBI Official Symbol: MRPS12
• NCBI Official Synonym Symbols: RPS12; RPMS12; RPSM12; MPR-S12; MT-RPS12
• NCBI Protein Information: 28S ribosomal protein S12, mitochondrial
• UniProt Protein Name: 28S ribosomal protein S12, mitochondrial
• Protein Family: 28S ribosomal protein
• UniProt Gene Name: MRPS12
• UniProt Synonym Gene Names: RPMS12; RPSM12; MRP-S12; S12mt
• UniProt Entry Name: RT12_HUMAN

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S12P family. The encoded protein is a key component of the ribosomal small subunit and controls the decoding fidelity and susceptibility to aminoglycoside antibiotics. The gene for mitochondrial seryl-tRNA synthetase is located upstream and adjacent to this gene, and both genes are possible candidates for the autosomal dominant deafness gene (DFNA4). Splice variants that differ in the 5' UTR have been found for this gene; all three variants encode the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

MRPS12: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S12P family. The encoded protein is a key component of the ribosomal small subunit and controls the decoding fidelity and susceptibility to aminoglycoside antibiotics. The gene for mitochondrial seryl-tRNA synthetase is located upstream and adjacent to this gene, and both genes are possible candidates for the autosomal dominant deafness gene (DFNA4). Splice variants that differ in the 5' UTR have been found for this gene; all three variants encode the same protein. [provided by RefSeq, Jul 2008]

Chromosomal Location of Human Ortholog: 19q13.1-q13.2

Cellular Component: small ribosomal subunit; mitochondrial inner membrane; mitochondrial ribosome

Molecular Function: protein binding; structural constituent of ribosome

Biological Process: mitochondrial translation; organelle organization and biogenesis