Rabbit anti-Human MRPL39 Polyclonal Antibody | anti-MRPL39 antibody

MRPL39, CT (MRPL39, C21orf92, MRPL5, RPML5, 39S ribosomal protein L39, mitochondrial, 39S ribosomal protein L5, mitochondrial) (Biotin)

Cat. Num. AAA6322177

• Gene Names: MRPL39; L39mt; MRPL5; RPML5; MRP-L5; PRED22; PRED66; MSTP003; C21orf92
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MRPL39; Polyclonal Antibody; CT (MRPL39; C21orf92; MRPL5; RPML5; 39S ribosomal protein L39; mitochondrial; 39S ribosomal protein L5; mitochondrial) (Biotin); anti-MRPL39 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-MRPL39 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MRPL39 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 301-330 amino acids from the C-terminal region of human MRPL39.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MRPL39 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the MRPL39 antibody detected the MRPL39 protein (arrow).)

Related Product Information for anti-MRPL39 antibody

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Two transcript variants encoding distinct isoforms have been described. A pseudogene corresponding to this gene is found on chromosome 5q.

Product Categories/Family for anti-MRPL39 antibody

Antibodies; Mitochondrial Proteins

References

Need, A.C., et al. Hum. Mol. Genet. 18(23):4650-4661(2009). Zhang, Z., et al. Genomics 81(5):468-480(2003). O'Brien, T.W. Gene 286(1):73-79(2002). Kenmochi, N., et al. Genomics 77 (1-2), 65-70 (2001): Spirina, O., et al. Gene 261(2):229-234(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 27886582
• NCBI GeneID: 54148
• NCBI Accession #: NP_059142.2; NP_542984.2
• NCBI GenBank Nucleotide #: NM_017446.3
• UniProt Accession #: Q9NYK5; Q32Q74; Q5QTR3; Q96Q65; Q9BSQ7; Q9BZV6; Q9NX44; C9JYA5
• Molecular Weight: 38,712 Da
• NCBI Official Full Name: 39S ribosomal protein L39, mitochondrial isoform a
• NCBI Official Synonym Full Names: mitochondrial ribosomal protein L39
• NCBI Official Symbol: MRPL39
• NCBI Official Synonym Symbols: L39mt; MRPL5; RPML5; MRP-L5; PRED22; PRED66; MSTP003; C21orf92
• NCBI Protein Information: 39S ribosomal protein L39, mitochondrial; L5mt; MRP-L39; 39S ribosomal protein L5, mitochondrial
• UniProt Protein Name: 39S ribosomal protein L39, mitochondrial
• Protein Family: 39S ribosomal protein
• UniProt Gene Name: MRPL39
• UniProt Synonym Gene Names: C21orf92; MRPL5; RPML5; L39mt; MRP-L39; L5mt; MRP-L5
• UniProt Entry Name: RM39_HUMAN

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Two transcript variants encoding distinct isoforms have been described. A pseudogene corresponding to this gene is found on chromosome 5q. [provided by RefSeq, Jul 2008]

Uniprot Description

MRPL39: 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Translation; Mitochondrial; Ribosomal

Chromosomal Location of Human Ortholog: 21q21.3

Cellular Component: mitochondrial inner membrane; mitochondrial ribosome

Molecular Function: nucleotide binding

Biological Process: mitochondrial translation; organelle organization and biogenesis