Rabbit anti-Human MIA40 Polyclonal Antibody | anti-MIA40 antibody

MIA40, CT (CHCHD4, MIA40, Mitochondrial intermembrane space import and assembly protein 40, Coiled-coil-helix-coiled-coil-helix domain-containing protein 4) (FITC)

Cat. Num. AAA6320704

• Gene Names: CHCHD4; MIA40; TIMM40
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MIA40; Polyclonal Antibody; CT (CHCHD4; Mitochondrial intermembrane space import and assembly protein 40; Coiled-coil-helix-coiled-coil-helix domain-containing protein 4) (FITC); CHCHD4; mitochondrial intermembrane space import and assembly protein 40; Coiled-coil-helix-coiled-coil-helix domain-containing protein 4; anti-MIA40 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-MIA40 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: MIA40 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-137 amino acids from the C-terminal region of human MIA40.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MIA40 Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the MIA40 antibody detected the MIA40 protein (arrow).)

Immunohistochemistry (IHC)

(MIA40 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MIA40 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-MIA40 antibody

CHCHD4, a component of human mitochondria, belongs to a protein family whose members share 6 highly conserved cysteine residues constituting a-CXC-CX(9)C-CX(9)C-motif in the C terminus (Hofmann et al., 2005 [PubMed 16185709]).

Product Categories/Family for anti-MIA40 antibody

Antibodies; Mitochondrial Proteins

References

Daithankar, V.N., et al. Biochemistry 48(22):4828-4837(2009). Chacinska, A., et al. J. Biol. Chem. 283(44):29723-29729(2008). Terziyska, N., et al. FEBS Lett. 581(6):1098-1102(2007). Hofmann, S., et al. J. Mol. Biol. 353(3):517-528(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 148612859
• NCBI GeneID: 131474
• NCBI Accession #: NP_001091972.1; NP_653237.1
• NCBI GenBank Nucleotide #: NM_001098502.1
• UniProt Accession #: Q8N4Q1; Q96AI2; Q96MY6; A8K3Z9
• Molecular Weight: 15,996 Da
• NCBI Official Full Name: mitochondrial intermembrane space import and assembly protein 40 isoform 1
• NCBI Official Synonym Full Names: coiled-coil-helix-coiled-coil-helix domain containing 4
• NCBI Official Symbol: CHCHD4
• NCBI Official Synonym Symbols: MIA40; TIMM40
• NCBI Protein Information: mitochondrial intermembrane space import and assembly protein 40; translocase of inner mitochondrial membrane 40 homolog; coiled-coil-helix-coiled-coil-helix domain-containing protein 4; mitochondrial intermembrane space import and assembly 40 homolog
• UniProt Protein Name: Mitochondrial intermembrane space import and assembly protein 40
• Protein Family: Mitochondrial intermembrane space import and assembly protein
• UniProt Gene Name: CHCHD4
• UniProt Synonym Gene Names: MIA40
• UniProt Entry Name: MIA40_HUMAN

NCBI Description

CHCHD4, a component of human mitochondria, belongs to a protein family whose members share 6 highly conserved cysteine residues constituting a -CXC-CX(9)C-CX(9)C- motif in the C terminus (Hofmann et al., 2005 [PubMed 16185709]).[supplied by OMIM, Mar 2008]

Uniprot Description

CHCHD4: Required for the import and folding of small cysteine- containing proteins (small Tim) in the mitochondrial intermembrane space (IMS). Probably acts by forming a redox cycle with GFER/ERV1 that involves a disulfide relay system. Precursor proteins to be imported into the IMS are translocated in their reduced form into the mitochondria. The oxidized form of MIA40 forms a transient intermolecular disulfide bridge with the reduced precursor protein, resulting in oxidation of the precursor protein that now contains an intramolecular disulfide bond and is able to undergo folding in the IMS (Probable). 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 3p25.1

Cellular Component: mitochondrion; mitochondrial intermembrane space

Molecular Function: protein disulfide oxidoreductase activity

Biological Process: 'de novo' posttranslational protein folding; protein maturation via protein folding; cellular protein metabolic process; protein import into mitochondrial intermembrane space; protein targeting to mitochondrion