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Western Blot (WB) (All lanes : Anti-MRPL10 Antibody at 1:1000 dilutionLane 1: A431 whole cell lysatesLane 2: HT-1080 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 29 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Rabbit anti-Human MRPL10 Polyclonal Antibody | anti-MRPL10 antibody

MRPL10 Antibody

Gene Names
MRPL10; L10MT; MRPL8; RPML8; MRP-L8; MRP-L10
Reactivity
Human
Applications
Western Blot, ELISA, Immunoprecipitation, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MRPL10; Polyclonal Antibody; MRPL10 Antibody; 39S ribosomal protein L10; mitochondrial; L10mt; MRP-L10; 39S ribosomal protein L8; L8mt; MRP-L8; MRPL8; RPML8; anti-MRPL10 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
0.01M PBS, pH 7.2, 0.1% Sodium azide, Glycerol 50%
Concentration
1mg/ml (varies by lot)
Sequence Positions
201-260
Sequence Length
271
Applicable Applications for anti-MRPL10 antibody
Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), Immunohistochemistry (IHC)-Paraffin
Application Notes
WB~~ 1:1000
Antigen Type
synthetic peptide
Antigen Source
KLH conjugated synthetic peptide derived from human MRPL10
Preparation and Storage
Store at -20 degree C.Stable for 12 months from date of receipt

Western Blot (WB)

(All lanes : Anti-MRPL10 Antibody at 1:1000 dilutionLane 1: A431 whole cell lysatesLane 2: HT-1080 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 29 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (All lanes : Anti-MRPL10 Antibody at 1:1000 dilutionLane 1: A431 whole cell lysatesLane 2: HT-1080 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 29 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
References
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Zody M.C.,et al.Nature 440:1045-1049(2006).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Kenmochi N.,et al.Genomics 77:65-70(2001).
Koc E.C.,et al.J. Biol. Chem. 276:43958-43969(2001).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
29283
NCBI Official Full Name
39S ribosomal protein L10, mitochondrial isoform a
NCBI Official Synonym Full Names
mitochondrial ribosomal protein L10
NCBI Official Symbol
MRPL10
NCBI Official Synonym Symbols
L10MT; MRPL8; RPML8; MRP-L8; MRP-L10
NCBI Protein Information
39S ribosomal protein L10, mitochondrial
UniProt Protein Name
39S ribosomal protein L10, mitochondrial
Protein Family
UniProt Gene Name
MRPL10
UniProt Synonym Gene Names
MRPL8; RPML8; L10mt; MRP-L10; L8mt; MRP-L8
UniProt Entry Name
RM10_HUMAN

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified three transcript variants that encode two different isoforms. A pseudogene corresponding to this gene is found on chromosome 5q. [provided by RefSeq, Nov 2010]

Uniprot Description

MRPL10: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified three transcript variants that encode two different isoforms. A pseudogene corresponding to this gene is found on chromosome 5q. [provided by RefSeq, Nov 2010]

Protein type: Ribosomal; Translation; Mitochondrial

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: nucleoplasm; mitochondrion; mitochondrial inner membrane; ribonucleoprotein complex; mitochondrial large ribosomal subunit

Molecular Function: structural constituent of ribosome

Biological Process: translation; mitochondrial translation; organelle organization and biogenesis; ribosome biogenesis and assembly

Research Articles on MRPL10

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Product Notes

The MRPL10 mrpl10 (Catalog #AAA9204185) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 201-260. The MRPL10 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MRPL10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), Immunohistochemistry (IHC)-Paraffin. WB~~ 1:1000. Researchers should empirically determine the suitability of the MRPL10 mrpl10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MRPL10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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