Rabbit MRE11A / MRE11 Polyclonal Antibody | anti-MRE11A antibody

Rabbit Polyclonal to Human MRE11A / MRE11

Cat. Num. AAA243722

• Gene Names: MRE11A; ATLD; HNGS1; MRE11; MRE11B
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: MRE11A / MRE11; Polyclonal Antibody; Rabbit Polyclonal to Human MRE11A / MRE11; Anti-MRE11A / MRE11 Antibody (N-Terminus) IHC-plus; MRE11A; AT-like disease; Endo/exonuclease Mre11; HNGS1; MRE11; MRE11 homolog 1; MRE11 homolog A; MRE11B; ATLD; Human MRE11A; anti-MRE11A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Human MRE11A
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 708

• Applicable Applications for anti-MRE11A antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB (1 - 2 ug/ml); Usage: MRE11 antibody can be used for detection of MRE11 by Western blot at 1-2 ug/ml.
• Target Species: Human
• Immunogen Description: A 14 amino acid peptide from near the amino terminus of human MRE11.
• Immunogen Type: Synthetic peptide
• Immunogen: MRE11A / MRE11 antibody was raised against a 14 amino acid peptide from near the amino terminus of human MRE11.
• Antigen Modification: N-Terminus
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-MRE11A / MRE11 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of MRE11 in rat lung tissue with MRE11 antibody at 20 ug/ml.)

Western Blot (WB)

(Western blot of MRE11 in rat lung tissue lysate with MRE11 antibody at (A) 1 and (B) 2 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 5031923
• NCBI GeneID: 4361
• NCBI Accession #: NP_005582.1
• NCBI GenBank Nucleotide #: NM_005591.3
• UniProt Accession #: P49959; O43475; B3KTC7
• Molecular Weight: 81,070 Da
• NCBI Official Full Name: double-strand break repair protein MRE11A isoform 1
• NCBI Official Synonym Full Names: MRE11 meiotic recombination 11 homolog A (S. cerevisiae)
• NCBI Official Symbol: MRE11A
• NCBI Official Synonym Symbols: ATLD; HNGS1; MRE11; MRE11B
• NCBI Protein Information: double-strand break repair protein MRE11A; AT-like disease; MRE11 homolog 1; MRE11 homolog A; endo/exonuclease Mre11; meiotic recombination 11 homolog 1; meiotic recombination 11 homolog A; DNA recombination and repair protein
• UniProt Protein Name: Double-strand break repair protein MRE11A
• Protein Family: Double-strand break repair protein
• UniProt Gene Name: MRE11A
• UniProt Synonym Gene Names: HNGS1; MRE11; MRE11 homolog 1; MRE11 homolog A
• UniProt Entry Name: MRE11_HUMAN

NCBI Description

This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

MRE11A: a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. Alternative splicing results in two different isoforms.

Protein type: DNA-binding; DNA repair, damage; Cell cycle regulation; Deoxyribonuclease

Chromosomal Location of Human Ortholog: 11q21

Cellular Component: nucleoplasm; chromosome, telomeric region; Mre11 complex; chromatin; nucleus; cytosol

Molecular Function: protein C-terminus binding; ATP-dependent DNA helicase activity; protein binding; nuclease activity; DNA binding; single-stranded DNA specific endodeoxyribonuclease activity; manganese ion binding; endonuclease activity; double-stranded DNA binding; 3'-5' exonuclease activity; endodeoxyribonuclease activity

Biological Process: positive regulation of kinase activity; sister chromatid cohesion; negative regulation of DNA endoreduplication; telomere maintenance via telomerase; regulation of mitotic recombination; DNA repair; DNA catabolic process, endonucleolytic; positive regulation of protein amino acid autophosphorylation; double-strand break repair via homologous recombination; DNA duplex unwinding; double-strand break repair via nonhomologous end joining; DNA recombination; cell proliferation; intra-S DNA damage checkpoint; nucleotide-excision repair; base-excision repair; double-strand break repair; mitotic cell cycle G2/M transition DNA damage checkpoint; meiotic recombination; synapsis; positive regulation of interferon type I production; innate immune response; response to DNA damage stimulus; telomere maintenance

Disease: Ataxia-telangiectasia-like Disorder 1