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Western Blot (WB) (Western blot analysis of mPR gamma expression in Hela (A), HGC27 (B), mouse kidney (C), mouse lung (D) whole cell lysates.)

Rabbit mPR gamma Polyclonal Antibody | anti-PAQR5 antibody

Anti-mPR gamma Antibody

Gene Names
PAQR5; MPRG
Reactivity
Human, Mouse, Sheep
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
mPR gamma; Polyclonal Antibody; Anti-mPR gamma Antibody; MPRG; Membrane progestin receptor gamma; Progestin and adipoQ receptor family member 5; Progestin and adipoQ receptor family member V; anti-PAQR5 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Sheep
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of mPR gamma protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.
Sequence Length
330
Applicable Applications for anti-PAQR5 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes
Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200); Immunofluorescence/Immunocytochemistry: (1/100 - 1/500)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human mPR gamma. The exact sequence is proprietary.
Preparation and Storage
Upon delivery aliquot and store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of mPR gamma expression in Hela (A), HGC27 (B), mouse kidney (C), mouse lung (D) whole cell lysates.)

Western Blot (WB) (Western blot analysis of mPR gamma expression in Hela (A), HGC27 (B), mouse kidney (C), mouse lung (D) whole cell lysates.)

Immunohistochemistry (IHC)

( Immunohistochemical analysis of mPR gamma staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Immunohistochemistry (IHC) ( Immunohistochemical analysis of mPR gamma staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Immunofluorescence (IF)

(Immunofluorescent analysis of mPR gamma staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)

Immunofluorescence (IF) (Immunofluorescent analysis of mPR gamma staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Related Product Information for anti-PAQR5 antibody
Rabbit polyclonal antibody to mPR gamma

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Membrane progestin receptor gamma
NCBI Official Synonym Full Names
progestin and adipoQ receptor family member 5
NCBI Official Symbol
PAQR5
NCBI Official Synonym Symbols
MPRG
NCBI Protein Information
membrane progestin receptor gamma
UniProt Protein Name
Membrane progestin receptor gamma
UniProt Gene Name
PAQR5
UniProt Synonym Gene Names
MPRG; mPR gamma
UniProt Entry Name
MPRG_HUMAN

Uniprot Description

PAQR5: Steroid membrane receptor. Binds progesterone. May be involved in oocyte maturation. Belongs to the ADIPOR family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 15q23

Cellular Component: integral to membrane; plasma membrane

Molecular Function: protein binding; steroid binding; steroid hormone receptor activity

Biological Process: multicellular organismal development; oogenesis; response to steroid hormone stimulus; steroid hormone mediated signaling

Research Articles on PAQR5

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Product Notes

The PAQR5 paqr5 (Catalog #AAA8242200) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-mPR gamma Antibody reacts with Human, Mouse, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's mPR gamma can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC). Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200); Immunofluorescence/Immunocytochemistry: (1/100 - 1/500). Researchers should empirically determine the suitability of the PAQR5 paqr5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "mPR gamma, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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