Western Blot (WB)
(MPP3 Antibody (C-term) western blot analysis in K562,NCI-H292 cell line lysates (35ug/lane).This demonstrates the MPP3 antibody detected the MPP3 protein (arrow).)
Rabbit anti-Human MPP3 Polyclonal Antibody | anti-MPP3 antibody
MPP3, CT (MPP3, DLG3, MAGUK p55 subfamily member 3, Discs large homolog 3, Protein MPP3) (PE)
Gene Names
MPP3; DLG3
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
MPP3; Polyclonal Antibody; CT (MPP3; DLG3; MAGUK p55 subfamily member 3; Discs large homolog 3; Protein MPP3) (PE); Protein MPP3; anti-MPP3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-MPP3 antibody
Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
IF: 1:10-50
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
IF: 1:10-50
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
MPP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 405-434 amino acids from the C-terminal region of human MPP3.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(MPP3 Antibody (C-term) western blot analysis in K562,NCI-H292 cell line lysates (35ug/lane).This demonstrates the MPP3 antibody detected the MPP3 protein (arrow).)
Western Blot (WB)
(MPP3 Antibody (C-term) western blot analysis in K562,NCI-H292 cell line lysates (35ug/lane).This demonstrates the MPP3 antibody detected the MPP3 protein (arrow).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of MPP3 Antibody (C-term) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of MPP3 Antibody (C-term) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
Flow Cytometry (FC/FACS)
(MPP3 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(MPP3 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-MPP3 antibody
This gene product is a member of a family of membrane-associated proteins termed MAGUKs (membrane-associated guanylate kinase homologs). MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. This protein contains a conserved sequence, called the SH3 (src homology 3) motif, found in several other proteins that associate with the cytoskeleton and are suspected to play important roles in signal transduction. Alternatively spliced transcript variants have been identified. One transcript variant is experimentally supported, but it doesn't encode a protein.
Product Categories/Family for anti-MPP3 antibody
References
Qin, L., et al. Zhonghua Gan Zang Bing Za Zhi 15(7):509-512(2007). Kantardzhieva, A., et al. FEBS J. 273(6):1152-1165(2006). Stelzl, U., et al. Cell 122(6):957-968(2005). Fukuhara, H., et al. Oncogene 22(40):6160-6165(2003). Shin, B.K., et al. J. Biol. Chem. 278(9):7607-7616(2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
66,152 Da
NCBI Official Full Name
MAGUK p55 subfamily member 3
NCBI Official Synonym Full Names
membrane protein, palmitoylated 3 (MAGUK p55 subfamily member 3)
NCBI Official Symbol
MPP3
NCBI Official Synonym Symbols
DLG3
NCBI Protein Information
MAGUK p55 subfamily member 3; discs large homolog 3; discs, large homolog 3; membrane protein palmitoylated 3
UniProt Protein Name
MAGUK p55 subfamily member 3
Protein Family
UniProt Gene Name
MPP3
UniProt Synonym Gene Names
DLG3
UniProt Entry Name
MPP3_HUMAN
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Product Notes
The MPP3 mpp3 (Catalog #AAA6321943) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MPP3, CT (MPP3, DLG3, MAGUK p55 subfamily member 3, Discs large homolog 3, Protein MPP3) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MPP3 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 IF: 1:10-50 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MPP3 mpp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MPP3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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