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Rabbit MOTA Polyclonal Antibody | anti-MOTA antibody

Rabbit anti-Escherichia coli (strain K12) MOTA Polyclonal Antibody

Gene Names
motA; ECK1891; flaJ; JW1879
Reactivity
E Coli (strain K12)
Applications
ELISA, Western Blot
Purity
Protein A/G
Synonyms
MOTA; Polyclonal Antibody; Rabbit anti-Escherichia coli (strain K12) MOTA Polyclonal Antibody; MOTA Antibody; Motility protein A; Chemotaxis protein MotA; motA flaJ b1890 JW1879; anti-MOTA antibody
Ordering
Host
Rabbit
Reactivity
E Coli (strain K12)
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Protein A/G
Form/Format
Liquid
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH7.4
Applicable Applications for anti-MOTA antibody
ELISA (EIA), WB (Western Blot) (ensure identification of antigen)
Immunogen
Recombinant Escherichia coli (strain K12) motA protein
Conjugation
Non-conjugate
Modification
Unmodified
Deliverables
1. 200ug recombinant immunogen protein/peptide (positive control).
2. 1mL preimmune serum (negative control)
3. 10mg rabbit polyclonal antibody purified by Protein A/G.
Quality Guarantee
1. Antibody purity >90% confirmed by SDS-PAGE.
2. Antibody titer > 1: 64,000 confirmed by ELISA
3. Western Blot positive for the immunogen protein (Not applicable for synthetic peptide).
Complementary Antibody
Customer can choose 1 FREE antibody from the list below. Please specify the catalog # when order:
* 6*His Monoclonal Antibody #MBS1491670
* DYKDDDDK tag Monoclonal Antibody #MBS1493946
* GAPDH Monoclonal Antibody #MBS1495524
* GFP Monoclonal Antibody #MBS1496050
* Myc tag Monoclonal Antibody #MBS1497694
* GST Monoclonal Antibody #MBS1497871
* Sumo tag Monoclonal Antibody #MBS1498878
* Goat Anti-Rabbit IgG (H+L) Antibody; HRP conjugated #MBS7053510
* HA-Tag Monoclonal Antibody #MBS7135631
* E-Tag Monoclonal Antibody #MBS7135632
* TUBA1A Monoclonal Antibody #MBS7135645
* TUBB Monoclonal Antibody #MBS9018332
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
32,011 Da
NCBI Official Full Name
proton conductor component of flagella motor
NCBI Official Symbol
motA
NCBI Official Synonym Symbols
ECK1891; flaJ; JW1879
NCBI Protein Information
proton conductor component of flagella motor
UniProt Protein Name
Motility protein A
Protein Family
UniProt Gene Name
motA
UniProt Synonym Gene Names
flaJ
UniProt Entry Name
MOTA_ECOLI

NCBI Description

Flagellar regulon. [More information is available at EcoGene: EG10601]. MotA and MotB comprise the stator element of the flagellar motor complex. [More information is available at EcoCyc: EG10601].

Uniprot Description

Function: MotA and MotB comprise the stator element of the flagellar motor complex. Required for rotation of the flagellar motor. Probable transmembrane proton channel. Overexpression of MotA, with or without MotB, restores motility in a yhjH disruption, (a c-di-GMP phosphodiesterase) suggesting there is an interaction (direct or indirect) between the c-di-GMP-binding flagellar brake protein YcgR and the flagellar stator. Ref.10

Subunit structure: Each stator complex is composed of 4 MotA and 2 MotB subunits; in E.coli 11 to 12 stator complexes can be involved in flagellar rotation. 2 A subunits and 1 B subunit are thought to form a single ion channel, so that each stator complex contains two channels. Interacts with FliG. Interacts with flagellar brake protein YcgR in the flagellar basal bodies (Ref.9). In another study (Ref.10) YcgR was not seen to interact with MotA, but instead with FliM and FliG, also in the flagellar basal body. Ref.9

Subcellular location: Cell inner membrane; Multi-pass membrane protein.

Sequence similarities: Belongs to the MotA family.

Research Articles on MOTA

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Product Notes

The MOTA mota (Catalog #AAA7162471) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Escherichia coli (strain K12) MOTA Polyclonal Antibody reacts with E Coli (strain K12) and may cross-react with other species as described in the data sheet. AAA Biotech's MOTA can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), WB (Western Blot) (ensure identification of antigen). Researchers should empirically determine the suitability of the MOTA mota for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MOTA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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