Western Blot (WB)
(Western blot analysis of extracts of C6 cells, using MonoMethyl-UHRF1-K385 antibody at 1:1000 dilution.C6 cells were treated by MG132(50 uM) at 37℃ for 90 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
Rabbit MonoMethyl-UHRF1-K385 Polyclonal Antibody | anti-MonoMethyl-UHRF1-K385 antibody
MonoMethyl-UHRF1-K385 Rabbit pAb
Gene Names
UHRF1; Np95; hNP95; ICBP90; RNF106; hUHRF1
Reactivity
Human, Mouse, Rat
Applications
Dot Blot, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Synonyms
MonoMethyl-UHRF1-K385; Polyclonal Antibody; MonoMethyl-UHRF1-K385 Rabbit pAb; UHRF1; ICBP90; Np95; RNF106; TDRD22; hNP95; hUHRF1; huNp95; anti-MonoMethyl-UHRF1-K385 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Sequence
KAKMA
Applicable Applications for anti-MonoMethyl-UHRF1-K385 antibody
Dot Blot (DB), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
DB: 1:500-1:2000
WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:50-1:200
WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:50-1:200
Immunogen
A synthetic monomethylated peptide around K385 of human UHRF1 (NP_001041666.1).
Cellular Location
Nucleus
Positive Samples
C6
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of C6 cells, using MonoMethyl-UHRF1-K385 antibody at 1:1000 dilution.C6 cells were treated by MG132(50 uM) at 37℃ for 90 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
Western Blot (WB)
(Western blot analysis of extracts of C6 cells, using MonoMethyl-UHRF1-K385 antibody at 1:1000 dilution.C6 cells were treated by MG132(50 uM) at 37℃ for 90 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
Dot Blot (DB)
(Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-UHRF1-K385 antibody at 1:1000 dilution.)
Dot Blot (DB)
(Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-UHRF1-K385 antibody at 1:1000 dilution.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U2OS cells using MonoMethyl-UHRF1-K385 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U2OS cells using MonoMethyl-UHRF1-K385 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
Related Product Information for anti-MonoMethyl-UHRF1-K385 antibody
Background: This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. It is regarded as a hub protein for the integration of epigenetic information. This gene is up-regulated in various cancers, and it is therefore considered to be a therapeutic target. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene exists on chromosome 12.
Product Categories/Family for anti-MonoMethyl-UHRF1-K385 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed Molecular Weight The band size is ~90kDa
NCBI Official Full Name
E3 ubiquitin-protein ligase UHRF1 isoform 1
NCBI Official Synonym Full Names
ubiquitin-like with PHD and ring finger domains 1
NCBI Official Symbol
UHRF1
NCBI Official Synonym Symbols
Np95; hNP95; ICBP90; RNF106; hUHRF1
NCBI Protein Information
E3 ubiquitin-protein ligase UHRF1; huNp95; nuclear protein 95; RING finger protein 106; transcription factor ICBP90; nuclear zinc finger protein Np95; inverted CCAAT box-binding protein of 90 kDa; ubiquitin-like, containing PHD and RING finger domains, 1;
UniProt Protein Name
E3 ubiquitin-protein ligase UHRF1
UniProt Gene Name
UHRF1
UniProt Synonym Gene Names
ICBP90; NP95; RNF106; HuNp95; hNp95; hUHRF1
UniProt Entry Name
UHRF1_HUMAN
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Product Notes
The MonoMethyl-UHRF1-K385 uhrf1 (Catalog #AAA9142145) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MonoMethyl-UHRF1-K385 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MonoMethyl-UHRF1-K385 can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). DB: 1:500-1:2000 WB: 1:500-1:2000 IHC: 1:50-1:200 IF: 1:50-1:200. Researchers should empirically determine the suitability of the MonoMethyl-UHRF1-K385 uhrf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KAKMA. It is sometimes possible for the material contained within the vial of "MonoMethyl-UHRF1-K385, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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