Western Blot (WB)
(Western blot analysis of Mob3C using RAW264.7 whole cell lysates)
Rabbit Mob3C Polyclonal Antibody | anti-Mob3C antibody
Mob3C Antibody
Gene Names
MOB3C; MOB1E; MOBKL2C
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
Mob3C; Polyclonal Antibody; Mob3C Antibody; MOB kinase activator 3C; Mob1 homolog 2C; MOB1E; MOB3C; Mps One Binder kinase activator like 2C; anti-Mob3C antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Mob3C antibody detects endogenous levels of total Mob3C
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
216
Applicable Applications for anti-Mob3C antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide derived from human Mob3C
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of Mob3C using RAW264.7 whole cell lysates)
Western Blot (WB)
(Western blot analysis of Mob3C using RAW264.7 whole cell lysates)
Immunofluorescene (IF)
(MBS9602157 staining RAW264.7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescene (IF)
(MBS9602157 staining RAW264.7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-Mob3C antibody
Function: May regulate the activity of kinases.
Similarity: Belongs to the MOB1/phocein family.
Similarity: Belongs to the MOB1/phocein family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 22 kDa
Predicted: 26 kDa
Predicted: 26 kDa
NCBI Official Full Name
MOB kinase activator 3C isoform 2
NCBI Official Synonym Full Names
MOB kinase activator 3C
NCBI Official Symbol
MOB3C
NCBI Official Synonym Symbols
MOB1E; MOBKL2C
NCBI Protein Information
MOB kinase activator 3C
UniProt Protein Name
MOB kinase activator 3C
Protein Family
UniProt Gene Name
MOB3C
UniProt Synonym Gene Names
MOBKL2C
NCBI Description
The protein encoded by this gene is similar to the yeast Mob1 protein. Yeast Mob1 binds Mps1p, a protein kinase essential for spindle pole body duplication and mitotic checkpoint regulation. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Uniprot Description
May regulate the activity of kinases.
Similar Products
Product Notes
The Mob3C mob3c (Catalog #AAA9602157) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Mob3C Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Mob3C can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the Mob3C mob3c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mob3C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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