Rabbit MMP9 Polyclonal Antibody | anti-MMP9 antibody

MMP9 Rabbit pAb

Cat. Num. AAA4757635

• Gene Names: MMP9; GELB; CLG4B; MMP-9; MANDP2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Affinity purified
• Synonyms: MMP9; Polyclonal Antibody; MMP9 Rabbit pAb; Matrix metalloproteinase-9; matrix metallopeptidase 9; GELB; CLG4B; MMP-9; MANDP2; anti-MMP9 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Produced by normal alveolar macrophages and granulocytes.
• Purity/Purification: Affinity purified
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Applicable Applications for anti-MMP9 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC1: 1:100-1:200; IF: 1:50-1:200
• Immunogen: A synthetic peptide of human MMP9
• Conjugation: Unconjugated
• Molecular Function: May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
• Modification: Unmodified
• Cell Position: Extracellular matrix, Secreted
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot - [KO Validated] MMP9 Polyclonal Antibody.Western blot analysis of extracts of various cell lines, using MMP9 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Exposure time: 10s.)

Western Blot (WB)

(Western blot - [KO Validated] MMP9 Polyclonal Antibody.Western blot analysis of extracts from normal (control) and MMP9 knockout (KO) 293T cells, using MMP9 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Exposure time: 90s.)

Immunofluorescence (IF)

(Immunofluorescence - [KO Validated] MMP9 Polyclonal Antibody. Immunofluorescence analysis of U-2OS cells using MMP9 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

Related Product Information for anti-MMP9 antibody

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008]
The accumulation of macrophages attenuates the effect of recombinant human endostatin on lung cancer

Product Categories/Family for anti-MMP9 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 74272287
• NCBI GeneID: 4318
• NCBI Accession #: NP_004985.2
• NCBI GenBank Nucleotide #: NM_004994.2
• UniProt Accession #: P14780; Q3LR70; Q8N725; Q9H4Z1; Q9UCJ9; Q9UCL1; Q9UDK2; B2R7V9
• Molecular Weight: 78,458 Da
• NCBI Official Full Name: matrix metalloproteinase-9 preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
• NCBI Official Symbol: MMP9
• NCBI Official Synonym Symbols: GELB; CLG4B; MMP-9; MANDP2
• NCBI Protein Information: matrix metalloproteinase-9; 92 kDa gelatinase; type V collagenase; macrophage gelatinase; 92 kDa type IV collagenase; matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
• UniProt Protein Name: Matrix metalloproteinase-9
• Protein Family: Matrix metalloproteinase
• UniProt Gene Name: MMP9
• UniProt Synonym Gene Names: CLG4B; MMP-9; GELB
• UniProt Entry Name: MMP9_HUMAN

NCBI Description

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008]

Uniprot Description

MMP9: May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. Exists as monomer or homodimer; disulfide-linked. Exists also as heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form. Interacts with ECM1. Activated by 4-aminophenylmercuric acetate and phorbol ester. Up-regulated by ARHGEF4, SPATA13 and APC via the JNK signaling pathway in colorectal tumor cells. Produced by normal alveolar macrophages and granulocytes. Inhibited by histatin-3 1/24 (histatin-5). Inhibited by ECM1. Belongs to the peptidase M10A family.

Protein type: Protease; EC 3.4.24.35; Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: proteinaceous extracellular matrix; extracellular space; extracellular region

Molecular Function: collagen binding; identical protein binding; protein binding; zinc ion binding; metalloendopeptidase activity; endopeptidase activity

Biological Process: positive regulation of keratinocyte migration; axon guidance; ossification; extracellular matrix organization and biogenesis; macrophage differentiation; positive regulation of apoptosis; proteolysis; extracellular matrix disassembly; collagen catabolic process; ephrin receptor signaling pathway; skeletal development; leukocyte migration; embryo implantation

Disease: Metaphyseal Anadysplasia 2