Western Blot (WB)
(Western blot analysis of extracts of mouse heart tissue, using MMP28 antibody.)
Rabbit MMP28 Polyclonal Antibody | anti-MMP28 antibody
MMP28 Antibody
Gene Names
MMP28; MM28; MMP25; MMP-25; MMP-28; EPILYSIN
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
MMP28; Polyclonal Antibody; MMP28 Antibody; Epilysin; Matrix metalloproteinase 28; Matrix metalloproteinase-28; MM 28; MM28; MMP 25; MMP 28; MMP-28; MMP25; MMP28_HUMAN; anti-MMP28 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
MMP28 antibody detects endogenous levels of MMP28.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
130
Applicable Applications for anti-MMP28 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human MMP28.
Subcellular Location
Secreted > Extracellular Space > Extracellular Matrix.
Tissue Specificity
Expressed at high levels in testes and lung. Low levels are detected in kidney, pancreas and skin. Also expressed in fetal lung, brain, skeletal muscle and kidney. Expressed selectively in keratinocytes. Widely expressed in several carcinomas as well. Is up-regulated in response to injury in the skin.
Predicted Cross Reactivity
Bovine, Horse, Sheep, Dog
Similarity
Bovine (91%), Horse (100%), Sheep (91%), Dog (91%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts of mouse heart tissue, using MMP28 antibody.)
Western Blot (WB)
(Western blot analysis of extracts of mouse heart tissue, using MMP28 antibody.)
Immunohistochemistry (IHC)
(MBS9603651 at 1/100 staining Human pancreas tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9603651 at 1/100 staining Human pancreas tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-MMP28 antibody
Function: Can degrade casein. Could play a role in tissues homeostasis and repair.
Post-translational Modifications: The precursor is cleaved by a furin endopeptidase.
Similarity: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. Belongs to the peptidase M10A family.
Post-translational Modifications: The precursor is cleaved by a furin endopeptidase.
Similarity: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. Belongs to the peptidase M10A family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 48 kDa
Predicted: 59 kDa
Predicted: 59 kDa
NCBI Official Full Name
matrix metalloproteinase-28 isoform 3
NCBI Official Synonym Full Names
matrix metallopeptidase 28
NCBI Official Symbol
MMP28
NCBI Official Synonym Symbols
MM28; MMP25; MMP-25; MMP-28; EPILYSIN
NCBI Protein Information
matrix metalloproteinase-28
UniProt Protein Name
Matrix metalloproteinase-28
Protein Family
UniProt Gene Name
MMP28
UniProt Synonym Gene Names
MMP25; MMP-28
NCBI Description
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix for both normal physiological processes, such as embryonic development, reproduction and tissue remodeling, and disease processes, such as asthma and metastasis. This gene encodes a secreted enzyme that degrades casein. Its expression pattern suggests that it plays a role in tissue homeostasis and in wound repair. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Apr 2014]
Uniprot Description
Can degrade casein. Could play a role in tissues homeostasis and repair.
Research Articles on MMP28
Similar Products
Product Notes
The MMP28 mmp28 (Catalog #AAA9603651) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MMP28 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MMP28 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the MMP28 mmp28 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MMP28, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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