anti-Human Coagulation Factor III Antibody | anti-F3 antibody

Coagulation Factor III (Ab-290) Antibody

Cat. Num. AAA8509629

• Gene Names: F3; TF; TFA; CD142
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: Coagulation Factor III; Coagulation Factor III (Ab-290) Antibody; CD142 antigen; F3; Tissue factor; coagulation factor III; thromboplastin; anti-F3 antibody

• Reactivity: Human
• Specificity: Coagulation Factor III (Ab-290) antibody detects endogenous levels of total Coagulation Factor III protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 238

• Applicable Applications for anti-F3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:1000
• Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human Coagulation Factor III around the phosphorylation site ofserine 290 (E-N-SP-P-L).
• Preparation and Storage: Store at-20 degree C for 1 year.

Western Blot (WB)

(Western blot analysis of extracts from COS7 cells and 293 cells, using Coagulation Factor III (Ab-290) antibody.)

References

Scarpati E.M., Biochemistry 26:5234-5238(1987). Morrissey J.H., Cell 50:129-135(1987). Spicer E.K., Proc. Natl. Acad. Sci. U.S.A. 84:5148-5152(1987).

NCBI and Uniprot Product Information

• NCBI GI #: 296010912
• NCBI GeneID: 2152
• NCBI Accession #: NP_001171567.1
• NCBI GenBank Nucleotide #: NM_001178096.1
• UniProt Accession #: P13726; Q6FHG2; Q86WH4; D3DT47
• Molecular Weight: 27,145 Da
• NCBI Official Full Name: tissue factor isoform 2
• NCBI Official Synonym Full Names: coagulation factor III, tissue factor
• NCBI Official Symbol: F3
• NCBI Official Synonym Symbols: TF; TFA; CD142
• NCBI Protein Information: tissue factor
• UniProt Protein Name: Tissue factor
• Protein Family: Tissue factor
• UniProt Gene Name: F3
• UniProt Synonym Gene Names: TF

NCBI Description

This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Uniprot Description

Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.