Rabbit anti-Human, Mouse MME Polyclonal Antibody | anti-MME antibody

MME, ID (MME, EPN, Neprilysin, Atriopeptidase, Common acute lymphocytic leukemia antigen, Enkephalinase, Neutral endopeptidase 24.11, Skin fibroblast elastase, CD10) (Biotin)

Cat. Num. AAA6321275

• Gene Names: MME; NEP; SFE; CD10; CALLA; CMT2T
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MME; Polyclonal Antibody; ID (MME; EPN; Neprilysin; Atriopeptidase; Common acute lymphocytic leukemia antigen; Enkephalinase; Neutral endopeptidase 24.11; Skin fibroblast elastase; CD10) (Biotin); CD10; anti-MME antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-MME antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: MME antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 728-755 amino acids from the Central region of human MME.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MME Antibody (Center) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the MME antibody detected the MME protein (arrow).)

Immunohistochemistry (IHC)

(MME Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MME Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-MME antibody

This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the identification of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. This gene, which encodes a 100-kD type II transmembrane glycoprotein, exists in a single copy of greater than 45 kb. The 5' untranslated region of this gene is alternatively spliced, resulting in four separate mRNA transcripts. The coding region is not affected by alternative splicing. [provided by RefSeq].

Product Categories/Family for anti-MME antibody

Antibodies; Elastase

References

Wang, S., et al. J. Neurochem. 115(1):47-57(2010). Ikenaga, N., et al. Gastroenterology 139(3):1041-1051(2010). Kim, H.S., et al. Histopathology 56(6):708-719(2010). Toussaint, J., et al. PLoS ONE 5 (8) (2010): Cui, L., et al. PLoS ONE 5 (8), E12121 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 116256327
• NCBI GeneID: 4311
• NCBI Accession #: NP_009218.2; NP_000893.2; NP_009219.2; NP_009220.2
• NCBI GenBank Nucleotide #: NM_007287.2
• UniProt Accession #: P08473; Q3MIX4; A8K6U6; D3DNJ9
• Molecular Weight: 85,514 Da
• NCBI Official Full Name: neprilysin
• NCBI Official Synonym Full Names: membrane metallo-endopeptidase
• NCBI Official Symbol: MME
• NCBI Official Synonym Symbols: NEP; SFE; CD10; CALLA; CMT2T
• NCBI Protein Information: neprilysin
• UniProt Protein Name: Neprilysin
• Protein Family: Neprilysin
• UniProt Gene Name: MME
• UniProt Synonym Gene Names: EPN; CALLA; NEP; Neutral endopeptidase; SFE
• UniProt Entry Name: NEP_HUMAN

NCBI Description

This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. This gene, which encodes a 100-kD type II transmembrane glycoprotein, exists in a single copy of greater than 45 kb. The 5' untranslated region of this gene is alternatively spliced, resulting in four separate mRNA transcripts. The coding region is not affected by alternative splicing. [provided by RefSeq, Jul 2008]