Rabbit anti-Human MLLT7 Polyclonal Antibody | anti-MLLT7 antibody

MLLT7, NT (Forkhead Box Protein O4, Fork Head Domain Transcription Factor AFX1, FOXO4, AFX, AFX1) (AP)

Cat. Num. AAA6477084

• Gene Names: FOXO4; AFX; AFX1; MLLT7
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: MLLT7; Polyclonal Antibody; NT (Forkhead Box Protein O4; Fork Head Domain Transcription Factor AFX1; FOXO4; AFX; AFX1) (AP); anti-MLLT7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MLLT7.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-MLLT7 antibody: Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 44-73 in the N-terminal region of human MLLT7, Swiss/UniProt Accession: P98177.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in Jurkat cell line lysates (35ug/lane) usingMBS629806. This demonstrates that MBS629806 detected the MLLT7 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue using MBS629806 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS629806 for immunohistochemistry.)

Product Categories/Family for anti-MLLT7 antibody

Antibodies; Transcription Factors

References

1. Crossley, L.J., J. Leukoc. Biol. 74(4):583-592 (2003). 2. Tang, T.T., et al., J. Biol. Chem. 278(32):30125-30135 (2003). 3. So, C.W., et al., Mol. Cell. Biol. 22(18):6542-6552 (2002). 4. Tang, T.T., et al., J. Biol. Chem. 277(16):14255-14265 (2002). 5. Kops, G.J., et al., Mol. Cell. Biol. 22(7):2025-2036 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 103472003
• NCBI GeneID: 4303
• NCBI Accession #: NP_005929
• NCBI GenBank Nucleotide #: NM_005938.3
• UniProt Accession #: P98177; O43821; Q13720; Q3KPF1; Q8TDK9; B7WPJ7
• Molecular Weight: 47,941 Da
• NCBI Official Full Name: forkhead box protein O4 isoform 1
• NCBI Official Synonym Full Names: forkhead box O4
• NCBI Official Symbol: FOXO4
• NCBI Official Synonym Symbols: AFX; AFX1; MLLT7
• NCBI Protein Information: forkhead box protein O4; fork head domain transcription factor AFX1; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 7
• UniProt Protein Name: Forkhead box protein O4
• UniProt Gene Name: FOXO4
• UniProt Synonym Gene Names: AFX; AFX1; MLLT7
• UniProt Entry Name: FOXO4_HUMAN

NCBI Description

This gene encodes a member of the O class of winged helix/forkhead transcription factor family. Proteins encoded by this class are regulated by factors involved in growth and differentiation indicating they play a role in these processes. A translocation involving this gene on chromosome X and the homolog of the Drosophila trithorax gene, encoding a DNA binding protein, located on chromosome 11 is associated with leukemia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]

Uniprot Description

FOXO4: transcription factor AFX1 containing 1 fork-head domain. May play a role in the insulin signaling pathway. Involved in acute leukemias by a chromosomal translocation t(X;11)(q13;q23) that involves MLLT7 and MLL/HRX. The result is a rogue activator protein. Two splice-variant isoforms have been described.

Protein type: DNA-binding; Oncoprotein; Transcription factor

Chromosomal Location of Human Ortholog: Xq13.1

Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; enzyme binding; DNA binding; sequence-specific DNA binding; transcription factor activity; transcription factor binding

Biological Process: epidermal growth factor receptor signaling pathway; transcription from RNA polymerase II promoter; muscle development; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; stem cell differentiation; negative regulation of smooth muscle cell differentiation; regulation of transcription from RNA polymerase II promoter; negative regulation of cell proliferation; negative regulation of angiogenesis; regulation of transcription, DNA-dependent; mitotic cell cycle G2/M transition DNA damage checkpoint; insulin receptor signaling pathway; innate immune response; cell cycle arrest